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细胞分析试剂盒
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中文名称

Tissue, Cartilage RNA Isolation Kit, BioAssay

英文名字
Tissue, Cartilage RNA Isolation Kit, BioAssay
供应商
USBiological
产品货号
T5595-0003
产品报价
¥1/1Kit
产品说明书
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产品描述
The Cartilage RNA Isolation Kit contains 4 solutions specifically for RNA isolation from different cartilage tissues. The isolated cartilage total RNA can be used for cDNA library construction, subtracted probe generation, RT-PCR, Northern blot analysis, primer extension, RNA protection assay, and In vitro translation. This kit can also be used for isolation of total RNA from other kinds of tissues.Kit Components:T5595-0003A: Solution 1, 1x50mlT5595-0003B: Phenol A, 1x50mlT5595-0003C: Solution 2, 1x6mlT5595-0003D: Solution 3, 1x50mlT5595-0003E: Solution 4, 1x14mlT5595-0003F: DEPC H2O/0.1mM EDTA, 1x50mlMaterials Required But Not Supplied:Isopropanol100% Ethanol70% EthanolChloroformStorage and Stability:Store T5595-0003A and T5595-0003B at 4°C. Store the rest of the kit components at room temperature. If precipitate forms in T5595-0003E, place the bottle in a 65°C water bath to dissolve it before use. Kit is stable for 6 months.Quality Control:Mouse Cartilage total RNA was isolated from each lot:1.The integrity of the RNA is examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA via electrophoreses on a denaturing agarose gel. 2. The quality and purity of total RNA were tested by spectrophotometer. A260/280 is between 1.8 and 2.0 (detected in 10mM Tris-Cl, pH 7.5). .3. The RNA is treated by DNase I, and is tested as DNA free RNA by PCR. 4. cDNA synthesis is successfully performed by using this RNA as template.
产品特点
针对Tissue, Cartilage RNA Isolation Kit, BioAssay该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到Tissue, Cartilage RNA Isolation Kit, BioAssay产品后将其置于RT/4°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
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