- 中文名称
Thyroxine (T4), Total, Human, BioAssay ELISA Kit
- 英文名字
- Thyroxine (T4), Total, Human, BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- T5460-09H
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 购买方式
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- 产品新闻
- 背景资料
- 产品描述
- Introduction:L-Thyroxine (T4) is a hormone that is synthesized and stored in the thyroid gland. Proteolytic cleavage of follicular thyroglobulin releases T4 into the bloodstream. Greater than 99% of T4 is reversibly bound to three plasma proteins in blood - thyroxine binding globulin (TBG) binds 70%, thyroxine binding pre-albumin (TBPA) binds 20%, and albumin binds 10%. Approximately 0.03% of T4 is in the free, unbound state in blood at any one time. Diseases affecting thyroid function may present a wide array of confusing symptoms. Measurement of total T4 by immunoassay is the most reliable and convenient screening test available to determine the presence of thyroid disorders in patients. Increased levels of T4 have been found in hyper-thyroidism due to Grave’s disease and Plummer’s disease and in acute and subacute thyroiditis. Low levels of T4 have been associated with congenital hypothyroidism, myxedema, chronic thryoiditis (Hashimoto’s disease), and with some genetic abnormalities.Intended Use:The Total Thyroxine (T4) competitive immunoenzyme assay kit is intended for the quantitative determination of total thyroxine (T4) concentration in human serum.Principle of the Test:In the T4 EIA, a certain amount of anti-T4 antibody is coated on microtiter wells. A measured amount of patient serum, and a constant amount of T4 conjugated with horseradish peroxidase are added to the microtiter wells. During incubation, T4 and conjugated T4 compete for the limited binding sites on the anti-T4 antibody. After a 60 minutes incubation at room temperature, the wells are washed 5 times by water to remove unbound T4 conjugate. A solution of TMB Reagent is then added and incubated for 20 minutes, resulting in the development of blue color. The color development is stopped with the addition of Stop Solution, and the absorbance is measured spectrophotometrically at 450nm. The intensity of the color formed is proportional to the amount of enzyme present and is inversely related to the amount of unlabeled T4 in the sample. By reference to a series of T4 standards assayed in the same way, the concentration of T4 in the unknown sample is quantified. Kit Components:T5460-09H1: Microtiter Plate,1x96wells. Coated with sheep anti-T4 antibody.T5460-09H2: Standard (0ug/dL), 1mlT5460-09H3: Standard (2ug/dL), 1mlT5460-09H4: Standard (5ug/dL), 1mlT5460-09H5: Standard (10ug/dL), 1mlT5460-09H6: Standard (15ug/dL), 1mlT5460-09H7: Standard (25ug/dL), 1mlT5460-09H8: Enzyme Conjugate Concentrate (11x), 1x1.3mlT5460-09H9: Enzyme Conjugate Diluent, 1x13mlT5460-09H10: TMB Reagent, 1x11mlT5460-09H11: Stop Solution, 1x11ml. 1N HClTest Principle:In the T4 EIA, a certain amount of anti-T4 antibody is coated on microtiter wells. A measured amount of patient serum, and a constant amount of T4 conjugated with horseradish peroxidase are added to themicrotiter wells. During incubation, T4 and conjugated T4 compete for the limited binding sites on the anti-T4 antibody. After a 60 minutes incubation at room temperature, the wells are washed 5 times by water to remove unbound T4 conjugate. A solution of TMB Reagent is then added and incubated for 20 minutes, resulting in the development of blue color. The color development is stopped with the addition of Stop Solution, and the absorbance is measured spectrophotometrically at 450nm. The intensity of the color formed is proportional to the amount of enzyme present and is inversely related to the amount of unlabeled T4 in the sample. By reference to a series of T4 standards assayed in the same way, the concentration of T4 in the unknown sample is quantified.Kit Components:Materials provided with the kit: Sheep anti-T4 coated microtiter wells, 96 wells.Standard (0ug/dL), 1x1mlStandard (2ug/dL), 1x1mlStandard (5ug/dL), 1x1mlStandard (10ug/dL), 1x1mlStandard (15ug/dL), 1x1mlStandard (25ug/dL), 1x1mlEnzyme Conjugate Concentrate (11), 1.3ml. Enzyme Conjugate Diluent, 13ml. TMB Reagent (One-Step), 11ml. Stop Solution (1N HCl), 11ml.Storage and Stability:Store all kit components at 4°C Kit is stable for 3 months after receipt.
- 产品特点
- 针对Thyroxine (T4), Total, Human, BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Thyroxine (T4), Total, Human, BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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