- 中文名称
Thyroid Stimulating Hormone, Human (TSH) BioAssay ELISA Kit
- 英文名字
- Thyroid Stimulating Hormone, Human (TSH) BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- T5400-45H
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 购买方式
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- 产品新闻
- 背景资料
- 产品描述
- Sample Type:SerumIntended Use:For the quantitative determination of thyroid stimulating hormone (TSH) concentration in human serum. The assay is useful in the diagnosis of thyroid or pituitary disorders.Introduction:The determination of serum or plasma levels of thyroid stimulating hormone (TSH or thyrotropin) is recognized as a sensitive method in the diagnosis of primary and secondary hypothyroidism (1). TSH is secreted by the anterior lobe of the pituitary gland and induces the production and release of thyroxine and triiodothyronine from the thyroid gland (2). It is a glycoprotein with a molecular weight of approximately 28,000D, consisting of two chemically different subunits, alpha and beta (3). Although the concentration of TSH in the blood is extremely low, it is essential for the maintenance of normal thyroid function. The release of TSH is regulated by a TSH-releasing hormone (TRH) produced by the hypothalamus. The levels of TSH and TRH are inversely related to the level of thyroid hormone. When there is a high level of thyroid hormone in the blood, less TRH is released by the hypothalamus, so less TSH is secreted by the pituitary. The opposite action will occur when there is decreased thyroid hormone in the blood. This process is known as a negative feedback mechanism and is responsible for maintaining the proper blood levels of these hormones (4,5). TSH and the pituitary glycoproteins: luteinizing hormone (LH), follicle-stimulating hormone (FSH), and human chorionic gonadotropin (hCG), have identical alpha chains. The beta chains are distinct but do contain regions with identical amino acid sequences. These regions of homology can cause considerable cross-reactivity with some polyclonal TSH antisera. The use of a monoclonal antibody in this TSH ELISA test eliminates such crossreactivity, which could result in falsely elevated TSH values in either menopausal or pregnant females -- a population whose evaluation of thyroid status is clinically significant (6,7,8).Test Principle:The TSH ELISA test is based on the principle of a solid phase enzyme-linked immunosorbent assay (9,10). The assay system utilizes a unique monoclonal antibody directed against a distinct antigenic determinant on the intact TSH molecule. Mouse monoclonal anti-TSH antibody is used for solid phase mobilization (on the microtiter wells). A goat anti-TSH antibody is in the antibodyenzyme (horseradish peroxidase) conjugate solution. The test sample is allowed to react simultaneously with the two antibodies, resulting in the TSH molecules being sandwiched between the solid phase and enzyme-linked antibodies. After a 60-minute incubation at room temperature, the wells are washed with water to remove unbound labeled antibodies. A solution of TMB Reagent is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of Stop Solution, changing the color to yellow. The concentration of TSH is directly proportional to the color intensity of the tests. Absorbance is measured spectrophotometrically at 450 nm.Kit Components:Murine Monoclonal Anti-TSH-coated microtiter wells.Standard (0IU/ml), 1x1 vialStandard, (0.5IU/ml), 1x1 vialStandard, (2IU/ml), 1x1 vialStandard, (5IU/ml), 1x 1vialStandard, (10IU/ml), 1x 1vialStandard, (25IU/ml), 1x1vial Enzyme Conjugate Reagent, 13mlTMB Reagent (One-Step), 11mlStop Solution (1N HCl), 11ml.Storage and Stability:Store all components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Thyroid Stimulating Hormone, Human (TSH) BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Thyroid Stimulating Hormone, Human (TSH) BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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