- 中文名称
Thrombin Heparin Cofactor II (T-HCII) Complexes BioAssay ELISA Kit (Antibodies only)
- 英文名字
- Thrombin Heparin Cofactor II (T-HCII) Complexes BioAssay ELISA Kit (Antibodies only)
- 供应商
- USBiological
- 产品货号
- T5045-75
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- 产品描述
- Thrombin-Heparin Cofactor II Complex:The activation of coagulation ultimately leads to the activation of prothrombin to the enzyme thrombin. Unless regulated, thrombin will act on its natural substrates that include fibrinogen, factor V, factor VIII, factor XIII, Protein C, TAFI as well as specific receptors on platelets and endothelial cells. The activity of thrombin in plasma is regulated in part through interaction with protease inhibitors. Based on kinetic rates and physiological concentrations, the primary inhibitor of thrombin in plasma is antithrombin (ATIII), followed by heparin cofactor II (HCII) and α2macroglobulin. The thrombin-heparin cofactor II complex (T-HCII) results when thrombin cleaves a scissile bond near the C-terminus of HCII, forming a covalent, 1:1 acyl enzyme intermediate with HCII with an apparent mass of 102 kD. Calcium is not required for this interaction, but the rate of thrombin inhibition by HCII can be accelerated 1000-fold by optimal concentrations of heparin. Unlike Antithrombin, thrombin inhibition by HCII is also enhanced by dermatan sulphate. T-HCII complexes are cleared from circulation by serpin-enzyme complex receptors on the surface of hepatocytes, with a half-life of 10 minutes (1-3).Principle of Sandwich-style ELISA:Affinity-purified antibody to human thrombin is coated onto the wells of a microtiter plate. Any remaining binding sites on the plastic wells are blocked with bovine serum albumin. The plates are washed and plasma or other fluids are applied. The coated antibody will capture the thrombin and thrombin-inhibitor complexes in the sample. After washing the plate to remove unbound material, a peroxidase conjugated second antibody to HCII is added to the plate to bind to the captured THCII complexes. After washing the plate to remove unbound conjugated antibody, the peroxidase activity is expressed by incubation with o-phenylenediamine (OPD). After a fixed development time the reaction is quenched with the addition of H2SO4 and the color produced is quantified using a microplate reader. The color generated is proportional to the concentration of THCII complex present in the sample.Quantity:Sufficient reagent for 5x96 well plates.Kit Components:1) Capture Antibody, 1x500ul, affinity purified Sheep antibody to Thrombin for coating plates.2) Detecting Antibody, 1x500ul, Peroxidase conjugated, affinity purified goat antibody to Heparin Cofactor II (HCII) for detection of HCII complexed to Thrombin.Storage and Stability:Store components at -20°C. Stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Thrombin Heparin Cofactor II (T-HCII) Complexes BioAssay ELISA Kit (Antibodies only)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Thrombin Heparin Cofactor II (T-HCII) Complexes BioAssay ELISA Kit (Antibodies only)产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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