- 中文名称
TRX1, Human, BioAssay ELISA Kit (Thioredoxin, Trx, ATL-derived Factor, ADF, Surface-associated Sulphydryl Protein, SASP, TXN, TRDX, TRX)
- 英文名字
- TRX1, Human, BioAssay ELISA Kit (Thioredoxin, Trx, ATL-derived Factor, ADF, Surface-associated Sulphydryl Protein, SASP, TXN, TRDX, TRX)
- 供应商
- USBiological
- 产品货号
- T5011-05M
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- 产品描述
- Thioredoxins(Trx) are small, multi-functional proteins with oxido-reductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cysteine residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor. The reduced Trx is a hydrogen donor for ribonucleotide reductase, the essential enzyme for DNA synthesis, and a potent general protein disulfide reductase with numerous functions in growth and redox regulations. Specific protein disulfide targets for reduction by Trx include protein disulfide –isomerase(PDI) and a number of transcription factors such as p53, NF-kB and AP-1 (T1-151).Trx is also capable of removing H2O2, particularly when it is coupled with either methionine sulfoxide reductase or several isoforms of peroxiredoxins. Trx is a multifunctional protein and has anti-inflammatory and antiapoptotic effects, as well as antioxidative effects. It is therefore feasible to think that Trx is a potential therapy for cardiac disease. Moreover, serum Trx is a well-recognized biomarker of various diseases involving oxidative stress, and this is also the case for cardiac disorders.Intended Use:Human Thioredoxin -1 (human Trx1) ELISA kit is to be used for the in vitro quantitative determination of human Trx1 in cell lysate and buffered solution. The assay will recognize both native and recombinant human Trx1. Sensitivity: 0.39ng/mlRange: 0.39-25ng/mlSpecificity:The following substances were tested and found to have no cross-reactivity: human Trx2, mouse Trx1 and rat Trx1.Test Principles:The design of this assay is based on a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to human Trx1. Samples are pippetted into these wells. Nonbound Trx1 and other components of the sample should be removed by washing, then biotin-conjugated monoclonal antibody specific to Trx1 added. In order to quantitatively determine the amount of Trx1 present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) should be added to each microplate well. The final step, a TMB-substrate solution added to each well. Finally, a sulfuric acid solution is added and the resulting yellow colored product is measured at 450nm. Since the increases in absorbency is directly proportional to the amount of captured Trx1.Kit Components:T5011-05M1: Microtiter Strips: 1x96 wellsT5011-05M2: Incubation Buffer: 1x30mlT5011-05M3: Washing Buffer, 20x: 2x25ml*T5011-05M4: Standard: 1x1vial lyophilizedT5011-05M5: Standard/Sample Dilution Buffer: 1x25mlT5011-05M6: Secondary Antibody (Biotin): 1x1vial lyophilizedT5011-05M7: Avidin (HRP), 100x: 1x150ulT5011-05M8: Secondary Antibody/HRP Dilution Buffer: 1x25mlT5011-05M9: TMB Substrate: 1x15mlT5011-05M10: Stop Solution, 1N H2SO4: 1x15mlStorage and Stability:Store *T5011-05M4 powder at 4°C liquid at -70°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对TRX1, Human, BioAssay ELISA Kit (Thioredoxin, Trx, ATL-derived Factor, ADF, Surface-associated Sulphydryl Protein, SASP, TXN, TRDX, TRX)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到TRX1, Human, BioAssay ELISA Kit (Thioredoxin, Trx, ATL-derived Factor, ADF, Surface-associated Sulphydryl Protein, SASP, TXN, TRDX, TRX)产品后将其置于4°C/-70°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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