- 中文名称
TXNRD1, Human, BioAssay ELISA Kit (Thioredoxin Reductase 1, Cytoplasmic, TR, Gene Associated with Retinoic and Interferon-induced Mortality 12 Protein, GRIM-12, Gene Associated with Retinoic and IFN-i
- 英文名字
- TXNRD1, Human, BioAssay ELISA Kit (Thioredoxin Reductase 1, Cytoplasmic, TR, Gene Associated with Retinoic and Interferon-induced Mortality 12 Protein, GRIM-12, Gene Associated with Retinoic and IFN-induced Mortality 12 Protein, KM-102-derived Reductase-like Factor, Thioredoxin Reductase TR1, GRIM12, KDRF)
- 供应商
- USBiological
- 产品货号
- T5010-14P
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- 产品描述
- The mammalian thioredoxin reductases(TrxRs) are a family of selenocysteine-containing pyridine nucleotide-disulfide oxido-reductases. Three different TrxR isoenzymes, TrxR1 as cytosolic, TrxR2 as mitochondrial, and TrxR3 as testis-specific thiol regulator are known. All the mammalian TrxRs are homologous to glutathione reductase with respect to primary structure including the conserved redox catalytic site (-Cys-Val-Asn-Val-Gly-Cys-) but distinctively with a C-terminal extension containing a catalytically active penultimate selenocysteine(SeCys) residue in the conserved sequence(-Gly-Cys-SeCys-Gly). TrxR is homodimeric protein in which each monomer includes an FAD prosthetic group, a NADPH binding site and a redox catalytic site. Electrons are transferred from NADPH via FAD and the active-site disulfide to C-terminal SeCys-containing redox center, which then reduces the substrate like thioredoxin. The members of TrxR family are 55–58 kD in molecular size and composed of three isoforms including cytosolic TrxR1, mitochondrial TrxR2, and TrxR3, known as Trx and GSSG reductase(TGR). TrxR plays a key role in protection of cells against oxidative stress and redox-regulatory mechanism of transcription factors and various biological phenomena. Many tumor cells have elevated TrxR levels and TrxR has been shown to play a major role in drug resistance. Inhibition of TrxR and its related redox reactions may thus contribute to a successful single, combinatory or adjuvant cancer therapy. A great number of effective natural and synthetic TrxR inhibitors are now available possessing antitumor potential ranging from induction of oxidative stress to cell cycle arrest and apoptosis.Intended Use:Human Thioredoxin Reductase-1 (human TR1) ELISA kit is to be used for the in vitro quantitative determination of human TR1 in human serum, human plasma, cell lysate and buffered solution. The assay will recognize both native and recombinant human TR1. This kit has been configured for research use only and is not to be used in diagnostic procedures.Sensitivity:The minimal detectable dose of human TR1 was calculated to be 0.39ng/ml, by subtracting two standard deviations from the mean of 10 zero standard replicates (ELISA buffer, S0) and intersecting this value with the standard curve obtained in the same calculation.Specificity:The following substances were tested and found to have no cross-reactivity: human TR2, mouse TR1 and rat TR1.Test Principle:The design of this assay is based on a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to human TR1. Samples are pippetted into these wells. Nonbound TR1 and other components of the sample should be removed by washing, then biotin-conjugated monoclonal antibody specific to TR1 added. In order to quantitatively determine the amount of TR1 present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) should be added to each microplate well. The final step, a TMB-substrate solution added to each well. Finally, a sulfuric acid solution is added and the resulting yellow colored product is measured at 450nm. Since the increases in absorbency is directly proportional to the amount of captured TR1.Kit Components:Microplate: 1x96 wellsIncubation buffer: 1x30mlWashing buffer, (10x): 1x100mlStandard protein: 1 glass vial lyophilizedStandard/sample dilution buffer: 1x25mlSecond antibody: 1 glass vial lyophilizedAV-HRP, (100x): 1x150ulSecondary antibody/AV-HRP dilution buffer: 1x25mlSubstrate (TMB): 1x20mlStop solution: 1x20mlStorage and Stability:Store components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对TXNRD1, Human, BioAssay ELISA Kit (Thioredoxin Reductase 1, Cytoplasmic, TR, Gene Associated with Retinoic and Interferon-induced Mortality 12 Protein, GRIM-12, Gene Associated with Retinoic and IFN-induced Mortality 12 Protein, KM-102-derived Reduc该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到TXNRD1, Human, BioAssay ELISA Kit (Thioredoxin Reductase 1, Cytoplasmic, TR, Gene Associated with Retinoic and Interferon-induced Mortality 12 Protein, GRIM-12, Gene Associated with Retinoic and IFN-induced Mortality 12 Protein, KM-102-derived Reductase-like Factor, Thioredoxin Reductase TR1, GRIM12, KDRF)产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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