- 中文名称
Testosterone, Free, Human, BioAssay ELISA Kit
- 英文名字
- Testosterone, Free, Human, BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- T2949-11
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- 产品描述
- The United States Biological Free Testosterone ELISA Bioassay is Competitive immunoenzymatic colorimetric method for quantitative determination of Free Testosterone concentration in serum and plasma. Free Testosterone (antigen) in the sample competes with horseradish peroxidase testosterone (enzyme-labeled antigen) for binding onto the limited number of anti-testosterone (antibody) sites on the microplates (solid phase). After incubation the bound/free separation is performed by a simple solid-phase washing. The enzyme substrate (H2O2) and the TMB-Substrate (TMB) are added. After an appropriate time has elapsed for maximum color development, the enzyme reaction is stopped and the absorbance is determinated. Free Testosterone concentration in the sample is calculated based on a series of standards. The color intensity is inversely proportional to the Free Testosterone concentration of in the sample. Testosterone in the blood is bound to SHBG (60 %) and in lower quantities bound to other proteins. The measurement of Free Testosterone (<1% of Total Testosterone) is the only estimating of the biologically active form of this hormone. Detection Range: 0-100pg/ml Sample size: 20ul Precision: Intra-assay: ≤10%Inter-assay: ≤10%Sensitivity: 0.6pg/ml Total Time: ~110 minKit Components: T2949-11A: Microtiter Plate, 1x96wellsT2949-11B: Standard (0pg/ml), 1x1mlT2949-11C: Standard (0.2pg/ml), 1x1mlT2949-11D: Standard (1.0pg/ml), 1x1mlT2949-11E: Standard (4.0pg/ml), 1x1mlT2949-11F: Standard (20.0pg/ml), 1x1mlT2949-11G: Standard (100.0pg/ml), 1x1mlT2949-11H: Control (0.84-2.03pg/ml), 1x1mlT2949-11J: HRP Conjugate, 1x15mlT2949-11K: Wash Buffer (10X), 1x50mlT2949-11L: TMB Substrate, 1x15mlT2949-11N: Stop Solution (0.15M H2SO4), 1x15ml Storage and Stability:Store all components at 4°C in the dark. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Diluted Wash Buffer is stable for 30 days at 4°C.
- 产品特点
- 针对Testosterone, Free, Human, BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Testosterone, Free, Human, BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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