- 中文名称
Scrub typhus, Human, IgM BioAssay ELISA Kit (Orientia tsutsugamushi, OT, Rickettsia)
- 英文名字
- Scrub typhus, Human, IgM BioAssay ELISA Kit (Orientia tsutsugamushi, OT, Rickettsia)
- 供应商
- USBiological
- 产品货号
- S8099-02
- 产品报价
- ¥1/1Kit

- 产品说明书
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- 购买方式
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- 产品新闻

- 背景资料
- 产品描述
- S8099-02 test for exposure to Orientia tsutsugamushi (OT; formerly Rickettsia) is an ELISA assay system for the detection of IgM antibodies in human serum to OT derived recombinant antigen (1-10). This test is to aid in the diagnosis of human exposure to OT species. It is not intended to screen blood or blood components, and is for research use only. Not for use in diagnostic procedures.Summary:Scrub Typhus is an infectious disease that is caused by Orientia tsutsugamushi (formerly Rickettsia), a tiny parasite about the size of bacteria that belongs to the family Rickettsiaceae. A bite from the larval trombiculid mite, a parasite of rodents, will transmit the disease. An ulcer of the skin is characteristic of a bite from a trombiculid mite, followed by symptoms including fever, a spotted rash on the torso, and swelling of the lymph glands. Scrub typhus generally occurs after exposure to areas with secondary (scrub) vegetation, which is where its name is derived from. However, the disease can also be prevalent in sandy, mountainous, and tropical areas. Scrub Typhus is a world wide illness, but particular to South East Asia and the Western Pacific. It accounts for approximately 20% of fever in some regions in South East Asia, where it is endemic. Illness lasts for a period of 10 to 12 days after the initial bite. With therapy, the fever will break within 36 hours, but if left untreated, complications or death may occur.Principle of the Test:The Scrub Typhus Detect ELISA system for IgM Test is a qualitative, membrane-based immunoassay for the detection of IgM antibodies to O. tsutsugamushi (OT) in serum, and plasma. Wells of each plate have been coated with unique recombinant antigen mix. During testing, the serum sample is diluted in sample diluent and applied to each well. After incubation and washing, the wells are treated with IgM enzyme conjugate (HRP). After a second incubation and washing step, the wells are incubated with the tetramethylbenzidine (TMB) substrate. An acidic stopping solution is then added and the degree of enzymatic turnover of the substrate is determined by absorbance measurement at 450nm. The absorbance measured is directly proportional to the concentration of IgM antibodies to OT present. A set of positive and negative controls are provided as internal controls. These are provided to monitor the integrity of the kit components.FactorToleranceNegative Control (NC) OD<0.200Positive Control (PC) OD>0.500Discrimination Capacity (RPC/NC)≥5.0Kit Components:S8099-02A: Microtiter Plate, 1x96 wellsS8099-02B: Sample Dilution Buffer, 2x25ml S8099-02C: Scrub Typhus IgM Positive Control, 1x50ul S8099-02D: Scrub Typhus IgM Negative Control, 1x50ulS8099-02E: Enzyme Conjugate (HRP), 1x12mlS8099-02F: Wash Buffer, 10X: 1x120ml S8099-02G: HRP Wash Solution, 1x20mlS8099-02H: TMB Substrate, 1x12ml S8099-02J: Stop Solution, 1x6ml Storage and Stability:Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Scrub typhus, Human, IgM BioAssay ELISA Kit (Orientia tsutsugamushi, OT, Rickettsia)该产品的特点优势,欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Scrub typhus, Human, IgM BioAssay ELISA Kit (Orientia tsutsugamushi, OT, Rickettsia)产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译,仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前,请浏览说明书确认。
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