- 中文名称
SOD3, Human, BioAssay ELISA Kit (Extracellular Superoxide Dismutase Cu-Zn] EC-SOD)
- 英文名字
- SOD3, Human, BioAssay ELISA Kit (Extracellular Superoxide Dismutase Cu-Zn] EC-SOD)
- 供应商
- USBiological
- 产品货号
- S8060-03M
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- 产品描述
- Superoxide dismutase (SOD) is an antioxidative enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O -) to hydrogen peroxide, which is then catalyzed to innocuous O and H O by 222 glutathione peroxidase and catalase. Three unique and highly compartmentalized mammalian superoxide dismutases have been biochemically and molecularly characterized to date. SOD1, or CuZn-SOD (EC 1.15.1.1), was the first enzyme to be characterized and is a copper and zinc- containing homodimer that is found almost exclusively in intracellular cytoplasmic spaces. SOD2, or Mn-SOD (EC 1.15.1.1), exists as a tetramer and is initially synthesized containing a leader peptide, which targets this manganese-containing enzyme exclusively to the mitochondrial spaces. SOD3, or EC-SOD (EC 1.15.1.1), is the most recently characterized SOD, exists as a copper and zinc-containing tetramer. SOD3, which is highly expressed in selected tissues including blood vessels, heart, lungs, kidney and placenta, is found in the extracellular matrix of tissues and is ideally situated to prevent cell and tissue damage initiated by extracellularly produced ROS. SOD3 contains a unique heparin-binding domain at its carboxy-terminus that establishes localization to the extracellular matrix where the enzyme scavenges superoxide anion. SOD3 plays an important role in maintaining vascular tone, attenuating age-related cognitive decline, lung function, and the metabolism of NO, and in the pathology of such diseases as atherosclerosis, diabetes, and arthritis.Intended Use:Human Superoxide Dismutase-3 (human SOD3) ELISA kit is to be used for the in vitro quantitative determination of human SOD3 in cell lysate and buffered solution. The assay will recognize both native and recombinant human SOD3. This kit has been configured for research use only and is not to be used in diagnostic procedures.Sensitivity:The minimal detectable dose of human SOD3 was calculated to be 2ng/ml, by subtractingtwo standard deviations from the mean of 10 zero standard replicates (ELISA buffer, S0) and intersecting this value with the standard curve obtained in the same calculation.Specificity:The following substances were tested and found to have no cross-reactivity: human SOD1, SOD2, SOD4.Test Principle:The design of this assay is based on a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to human SOD3. Samples are pippetted into these wells. Nonbound SOD3 and other components of the sample should be removed by washing, then biotin-conjugated monoclonal antibody specific to SOD3 added. In order to quantitatively determine the amount of SOD3 present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) should be added to each microplate well. The final step, a TMB-substrate solution added to each well. Finally, a sulfuric acid solution is added and the resulting yellow colored product is measured at 450nm. Since the increases in absorbency is directly proportional to the amount of captured SOD3.Kit Components:Microplate: 1x96 wellsIncubation buffer: 1x30mlWashing buffer, (10x): 1x100mlStandard protein: 1 glass vial lyophilizedStandard/sample dilution buffer: 1x25mlSecond antibody: 1 glass vial lyophilizedAV-HRP, (100x): 1x150ulSecondary antibody/AV-HRP dilution buffer: 1x25mlSubstrate (TMB): 1x20mlStop solution: 1x20mlStorage and Stability:Store components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对SOD3, Human, BioAssay ELISA Kit (Extracellular Superoxide Dismutase Cu-Zn] EC-SOD)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到SOD3, Human, BioAssay ELISA Kit (Extracellular Superoxide Dismutase Cu-Zn] EC-SOD)产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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