- 中文名称
Sulfate Assay Kit, BioAssay
- 英文名字
- Sulfate Assay Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- S8016-96
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- 产品描述
- Inorganic Sulfate is one of the most abundant anions in mammalian plasma. Sulfate plays important physiological roles in activating and detoxifying xenobiotics, steroids, neurotransmitters, and bile acids. Sulfate is needed for the biosynthesis of glycosaminoglycans, cerebroside sulfate, and heparin sulfate. Undersulfation of cartilage proteoglycans has been associated with human inherited osteochon- drodysplasia disorders. In mammals, sulfate homeostasis is regulated by the kidney. The majority of filtered sulfate is absorbed in the proximal tubules, and only 5–20% of the filtered load is excreted into the urine.Simple, direct and automation-ready procedures for quantitative determination of inorganic sulfate find wide applications in research and drug discovery. Sulfate assay kit is designed to measure sulfate concentration in biological fluids such as serum and urine. The improved method utilizes the quantitative formation of insoluble barium sulfate in polyethylene glycol. The turbidity measured between 540 and 610nm is proportional to sulfate level in the sample.Key Features:Sensitive and accurate. Detection range 0.02mM (0.19 mg/dL) to 2mM (19.2 mg/dL) sulfate in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min.Applications:Direct Assays: inorganic sulfate in serum and urine.Pharmacology: effects of drugs on sulfate metabolism.Kit Contents: (200 tests in 96-well plates) Reagent A: 25ml Reagent B: 2.4 g Powder TCA Reagent: 25mlSulfate Standard: 1ml 60mMStorage Conditions. The kit is shipped and stored at room temperature. Shelf life of three months after receipt.Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.Procedures:Sample Treatment: Urine samples should be diluted 10-fold in deionized water prior to assay. Fresh serum or plasma (non-hemolyzed) samples can be either assayed immediately, or frozen for future tests. Samples should be deproteinated as follows: mix 200ul sample and 100ul TCA Reagent in a 1.5-mL Eppendorf tube. Spin down protein precipitates 5 min at 14,000 rpm on a table centrifuge. Transfer 200ul supernatant for assay. Procedure using 96-well plate: 1. Standards. Prepare a 2.0mM Premix by mixing 20ul 60mM Sulfate Standard with 580ul dH2O. Dilute Premix as follows.NoPremix+H2OSulfate (mM)1200ul+0ul2.02100ul+100ul1.0350ul+150ul0.540ul+200ul0Transfer 200ul Standards into separate wells of a clear flat-bottom 96-well plate. Note: when deproteination is required (i.e. serum or plasma), treat the standards by adding 100ul TCA Reagent to 200ul of each standard, mix and transfer 200ul of the resulting standard into separate wells. Transfer 200ul Samples (see above for Sample Treatment) into separate wells of the plate.2. Working Reagent. The Working Reagent (WR) must be prepared fresh and used within 1 hour after reconstitution. Prepare enough WR for all samples and standards (100ul WR per assay well) by mixing 95 mg Reagent B perml Reagent A, i.e. 10 assay wells, mix 95 mg Reagent B with 1ml Reagent A. Vortex for at least 1 min to ensure complete dissolution of the powder and incubate the reconstituted Working Reagent for 10 min before use. Use a multi-channel pipettor, add 100ul Working Reagent to each assay well. Tap plate to mix well. If TCA precipitation was required, mixing the samples and standards with the WR can be improved by pipetting up and down once.3. Incubate 5 min at room temperature and read optical density at 540- 610nm (600nm).Calculation:Sulfate concentration (mM) in the sample can be calculated as follows, [Sulfate]=ODSAMPLE–ODH2O × n (mM)SlopeODSAMPLE and ODH2O are the OD values of the sample well and the water well (Standard #4), respectively. n=10 for urine. Conversions: 1mM sulfate equals 9.61 mg/dL or 96.1 ppm.General Considerations:This procedure can be scaled up to assays in a cuvette.The following compounds have been tested and do not interfere: 400mM sodium chloride, 500mM urea, 5mM sodium phosphate, 4mM sodium citrate, 1.5mM sodium EDTA. Ester sulfate can be determined following a digestion step (Lundquis, P. et al. (1980). Clin. Chem. 26:1178-81). This will allow quantification of total inorganic and ester sulfate in a sample.Materials Required, But Not Provided:Pipetting devices and accessories, clear flat-bottom 96-well plates and plate reader, or cuvets and spectrophotometer.Examples:Duplicate assays for rat serum, fetal bovine serum, human serum, and human urine gave sulfate content of 1.54 ± 0.07, 1.69 ± 0.09, 0.48 ± 0.01 and 10.5 ± 0.09mM (n=2).
- 产品特点
- 针对Sulfate Assay Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
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- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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