- 中文名称
STAT3, phosphorylated (Tyr705) ELISA Kit, BioAssay (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF)
- 英文名字
- STAT3, phosphorylated (Tyr705) ELISA Kit, BioAssay (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF)
- 供应商
- USBiological
- 产品货号
- S7969-90
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Phospho-Stat3 (Tyr705) Sandwich ELISA Kit is a solid phase sandwich enzymelinked immunosorbent assay (ELISA) that detects endogenous levels of Phospho-Stat3 (Tyr705) protein. A Stat3 rabbit monoclonal antibody has been coated onto the microwells. After incubation with cell lysates, both nonphospho-and phospho-Stat3 proteins are captured by the coated antibody. Following extensive washing, a phospho-Stat3 mouse monoclonal antibody is added to detect the captured phospho-Stat3 protein. HRP-linked anti-mouse antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of optical density for this developed color is proportional to the quantity of phospho-Stat3 protein. Stat3 is a key signaling molecule for many cytokines and growth-factor receptors (1), and is required for murine fetal development (2). Additionally, Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727, apparently via the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function. The relative expression levels of Stat3alpha (86kD) and Stat3b (79kD) depend on cell type, ligand exposure or maturation stage of the cells (10). It is notable that Stat3b lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).Kit Components:Stat3 Monoclonal Ab Coated Micrtiter plate, 1x96 wellsP-Stat3 (Y705) Monoclonal Detection Ab, 1x11mlAnti-mouse IgG, HRP-linked Antibody, 1x11mlTMB Substrate, 1x11mlSTOP Solution, 1x11mlELISA Wash Buffer (20X), 1x25mlELISA Sample Diluent, 1x25mlCell Lysis Buffer (10X), 1x15mlStorage and Stability:Kit should be stored at 4°C wtih the exception of 10X Cell Lysis Buffer, which is stored at -20°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对STAT3, phosphorylated (Tyr705) ELISA Kit, BioAssay (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到STAT3, phosphorylated (Tyr705) ELISA Kit, BioAssay (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
-
- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
-
