- 中文名称
ST2, BioAssay ELISA Kit (Mouse) (Interleukin-1 Receptor-like 1, Protein ST2, IL1RL1, DER4, T1)
- 英文名字
- ST2, BioAssay ELISA Kit (Mouse) (Interleukin-1 Receptor-like 1, Protein ST2, IL1RL1, DER4, T1)
- 供应商
- USBiological
- 产品货号
- S6606-50P
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Intended Use:The ST2, Mouse BioAssay ELISA Kit is a 4.5 hour solid phase ELISA designed to measure mouse ST2 in cell culture supernates, serum, and plasma. Test Principle:This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for mouse ST2 has been pre-coated onto a microplate. Standards, Control, and samples are pipetted into the wells and any mouse ST2 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked monoclonal antibody specific for mouse ST2 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells. The enzyme reaction yields a blue product that turns yellow when the Stop Solution is added. The intensity of the color measured is in proportion to the amount of mouse ST2 bound in the initial step. The sample values are then read off the standard curve.Kit Components:S6606-50P1: Microwell Strips, 1x96 wells*S6606-50P2: Standard (lyophilized), 1x1vial (50ng)*S6606-50P3: Control (lyophilized), 0.97-1.62ng/ml, 1x1vialS6606-50P4: HRP Conjugate, 1x12mlS6606-50P5: Assay Diluent, 1x12mlS6606-50P6: Calibrator Diluent, 1x21mlS6606-50P7: Wash Buffer, 25X, 1x50mlS6606-50P8: Color Reagent A, 1x12mlS6606-50P9: Color Reagent B, 1x12ml S6606-50P10: Stop Solution (HCl), 1x23mlStorage and Stability:Store components at 4°C. Stable for 6 months after receipt. Upon reconstitution of *S6606-50P2 & *S6606-50P3, aliquot and store at -20°C for up to 1 month. Avoid repeated freeze-thaw cycles. For maximum recovery of product, centrifuge the original vial prior to removing the cap.Sensitivity:Fifty-three assays were evaluated and the minimum detectable dose (MDD) of mouse ST2 ranged from 0.004-0.059ng/ml. The mean MDD was 0.014ng/ml.The MDD was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.Specificity:This assay recognizes both recombinant and natural mouse ST2. The factors listed below were prepared at 100ng/ml in Calibrator Diluent (1X) and assayed for crossreactivity. Preparations of the following factors prepared at 100ng/ml in a mid-range mouse ST2 control were assayed for interference. No significant crossreactivity or interference was observed.Recombinant mouse:IL-1a, IL-1b, IL-1ra, IL-1 R1, IL-1 R2, IL-1 RAPL2/IL-1 R9, IL-1 Rrp2/IL-1 R6, IL-18, IL-33 (aa1-108), SIGIRR, TLR1, TLR2, TLR3, TLR4, TLR5, TLR6, TLR7, TLR8, TLR12Recombinant mouse IL-33 (aa109-266) does not crossreact in this assay, but it does interfere at concentrations above 0.188ng/ml.Recombinant human:IL-1 RAcp/IL-1 R3, IL-33 (aa1-111), IL-33 (aa112-270), ST2/IL-1 R4, TIGIRR
- 产品特点
- 针对ST2, BioAssay ELISA Kit (Mouse) (Interleukin-1 Receptor-like 1, Protein ST2, IL1RL1, DER4, T1)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到ST2, BioAssay ELISA Kit (Mouse) (Interleukin-1 Receptor-like 1, Protein ST2, IL1RL1, DER4, T1)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
-
- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
-
