- 中文名称
Soy Protein Residue, BioAssay ELISA Kit
- 英文名字
- Soy Protein Residue, BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- S5365-20
- 产品报价
- ¥1/1Kit

- 产品说明书
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- 购买方式
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- 产品新闻

- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
- 产品描述
- The United States Biological Soy Protein Residue (in Food) ELISA is a highly sensitive detection system for the quantification of soy residues in cookies, cereals, ice cream, chocolate, instant soups and sausage.Background:Soybean (Glycine max) belongs to the legume family and contains a high level of protein (39%). Many of these proteins are known for being allergenic, such as Gly m1, Glycinin, Kunitz-Trypsin-Inhibitor and Gly m4 which is known to be cross reactive to birch pollen allergen Bet v1. For this reason soy represents an important food allergen. For soy allergic persons hidden soy allergens in food are a critical problem. Already very low amounts of soy can cause allergic reactions, which may lead to anaphylactic shock in severe cases. Because of this, soy allergic persons must strictly avoid the consumption of soy or soy-containing food. Partly undeclared addition of soy as additive in many foods is of particular importance. Cross-contaminations, mostly as a consequence of the production process, represent another problem. The chocolate production process is a representative example. Thus, sensitive detection systems for soy residues in foodstuffs are required. Only a few soy proteins are stable to conventional production processes (for example, high temperature). For this reason robust indicator proteins are necessary for detection of soy protein residue. Soy trypsin inhibitor (STI) is one such protein.This quantitative test is based on the principle of sandwich enzyme linked immunosorbent assay. An antibody directed against STI is bound on the surface of a microtiter plate. Soy-containing samples or standards are placed into the wells of the microtiter plate and incubated at room temperature. The wells are then washed to remove unbound material. A peroxidase-conjugated secondary antibody is added and the plate incubated and later washed. Substrate solution is added, resulting in the development of a blue color. Color development is halted by addition of the stop solution, which converts the color of the solution to yellow. The absorbance is measured photometrically at 450nm. The concentration of STI and hence, concentration of soy protein, is directly proportional to the color intensity of the sample.Sample required: 5gSensitivity: 16 parts per billionKit Components:S5365-20A: Microtiter Plate, 12 strips with 8 breakable wells. Coated with anti-STI antibody.S5365-20B1: Standard 1, 1x 1ml. 0ppb STI. Dyed red; ready to use.S5365-20B2: Standard 2, 1x 1ml. 40ppb STI. Dyed red; ready to use.S5365-20B3: Standard 3, 1x 1ml. 100ppb STI. Dyed red; ready to use.S5365-20B4: Standard 4, 1x 1ml. 400ppb STI. Dyed red; ready to use.S5365-20B4: Standard 5, 1x 1ml. 1000ppb STI. Dyed red; ready to use.S5365-20C: Conjugate (Anti-STI-Peroxidase), 1 x 15ml. Dyed red; ready to useS5365-20D: Substrate Solution (TMB): 1 x 15ml. Ready to useS5365-20E: Extraction and Sample Dilution Buffer (Tris), 10X: 2 x 120ml. Dyed red. S5365-20F: Washing Solution (PBS + Tween 20), 10X: 1 x 60ml. Storage and Stability: Store components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vials prior to removing the cap.
- 产品特点
- 针对Soy Protein Residue, BioAssay ELISA Kit 该产品的特点优势,欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Soy Protein Residue, BioAssay ELISA Kit 产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
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- 注意
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