- 中文名称
Signaling Nodes Multi-Target BioAssay ELISA Kit
- 英文名字
- Signaling Nodes Multi-Target BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- S1013-84
- 产品报价
- ￥1/1Kit
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- MultiTarget Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that combines the reagents necessary to detect endogenous levels of Akt1, phospho-Akt1 (Ser473), phospho-MEK1 (Ser217/221), phospho-p38a MAPK (Thr180/Tyr182), phospho-Stat3 (Tyr705) and phospho-NF-kB p65 (Ser536). These molecules represent convergence points and key regulatory proteins in signaling pathways controlling cellular events such as growth and differentiation, energy homeostasis and the response to stress and inflammation. Sixteen tests are provided for each target protein. Specific assay formulations for the indicated target proteins can be found in the datasheets associated with the individual PathScan® Sandwich ELISA Kits‡. Briefly, a capture antibody† has been coated onto the microwells. After incubation with cell lysates, the target protein is captured by the coated antibody. Following extensive washing, a detection antibody† is added to detect the captured target protein. An HRP-linked secondary antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of absorbance for this developed color is proportional to the quantity of bound target protein. Nodes Multi-Target Sandwich ELISA Ki detects endogenous levels of six proteins: Akt1, phospho-Akt1 (Ser473), phospho-MEK1 (Ser217/221), phospho-p38a MAPK (Thr180/Tyr182), phospho-Stat3 (Tyr705) and phospho-NF-kB p65 (Ser536). Differential phosphorylation of these proteins can be observed over time in response to various growth factor and cytokine treatments. As shown in Figure 1, stimulation of serum starved NIH/3T3 cells with PDGF generates robust phosphorylation of Akt1 at Ser473 and MEK1 at Ser217/221 but relatively weak phosphorylation of Stat3 at Tyr705. Simultaneous treatment of NIH/3T3 cells with TNF-a and IL-1β results in phosphorylation of Akt1 at Ser473, MEK1 at Ser217/221, p38a MAPK at Thr180/Tyr182 and NF-kB p65 at Ser536 but no activation of Stat3. By comparison, NIH/3T3 cells respond to IL-6 primarily through the activation of Stat3 rather than Akt1, MEK1, p38a MAPK or NF-kB p65. The level of each target protein (phospho and nonphospho) remains unchanged throughout the 80 minute time course as demonstrated by Western analysis.Akt is a protooncogene with a critical regulatory role in diverse cellular processes including growth, survival and the cell cycle. Akt is also a major regulator of insulin signaling and glucose metabolism (1-4). Akt is activated by PI3 kinase signaling and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation withing the carboxyl terminus at Ser473 by PDK2 (5-7). MEK1 and MEK2 are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation. Activation of MEK1 and MEK2 occurs through phosphorylation of serine 217 and serine 221 by Raf-like molecules. MEK activates p44 and p42 MAP kinase (8-10). p38 MAP kinase (MAPK) participates in a signaling cascade controlling the cellular response to pro-inflammatory cytokines and a variety of cellular stresses. MKK3, MKK6 and SEK (MKK4) activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182 (11-14). The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors. Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation and DNA binding (15,16).Kit Components:Akt Rabbit Ab Coated Microwells, 16 testsAkt1 Mouse Detection Ab, 1x1.8ml Anti-Mouse IgG HRP-Linked Ab, 1x1.8mlPhospho-Akt (Ser473) Rabbit Ab Coated Microwells, 16 testsAkt1 Mouse Detection Ab, 1x1.8mlAnti-Mouse IgG HRP-Linked Ab, 1x1.8mlMEK1 Mouse Ab Coated Microwells, 16 testsPhospho-MEK1/2 (Ser217/221) Rabbit Detection Ab, 1x1.8mlAnti-Rabbit IgG HRP-Linked Ab, 1x1.8mlp38α MAP Kinase Rabbit Ab Coated Microwells, 16 testsPhospho-p38α MAPK (Thr180/Tyr182) Mouse Detection Ab, 1x1.8mlAnti-Mouse IgG HRP-Linked Ab, 1x1.8mlStat3 Rabbit Ab Coated Microwells, 16 testsPhospho-Stat3 (Tyr705) Mouse Detection Ab, 1.8mlAnti-Mouse IgG HRP-Linked Ab, 1x1.8mlNF-κB p65 Mouse Ab Coated Microwells, 16 testsPhospho-NF-κB p65 (Ser536) Rabbit Detection Ab, 1x1.8mlAnti-Rabbit IgG HRP-Linked Ab, 1x1.8mlTMB Substrate, 1x11mlSTOP Solution, 1x11ml20X Wash Buffer, 1x25mlSample Diluent, 1x25mlCell Lysis Buffer (10X),1x15mlStorage and Stability:Store components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Storage and Stability:Store components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Signaling Nodes Multi-Target BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Signaling Nodes Multi-Target BioAssay ELISA Kit产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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