- 中文名称
SAM Methyltransferase Assay Kit, Colorimetric , BioAssay
- 英文名字
- SAM Methyltransferase Assay Kit, Colorimetric , BioAssay
- 供应商
- USBiological
- 产品货号
- S0070-84
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Methylation of key biological molecules and proteins plays important roles in numerous biological systems, including signal transduction, biosynthesis, protein repair, gene silencing, and chromatin regulation (1). The S-adenosylmethionine (SAM) dependent methyltransferases use SAM, the second most commonly used enzymatic cofactor after ATP. SAM, also known as AdoMet, acts as a donor of a methyl group that is required for the modification of proteins and DNA (2). Aberrant levels of SAM have been linked to many abnormalities, including Alzheimer’s Disease, depression, Parkinson’s Disease, Multiple Sclerosis, liver failure, and cancer (1,2).Colorimetric SAM Methyltransferase BioAssay is a continuous enzyme-coupled assay that can continuously monitor SAM-dependent methyltransferases (3). Figure 1 outlines the general scheme of the assay. The removal of the methyl group from SAM generates S-adenosylhomocysteine (AdoHcy), which is rapidly converted to S-ribosylhomocysteine and adenine by AdoHcy nucleosidase. This rapid conversion prevents the buildup of AdoHcy and its feedback inhibition on the methylation reaction. Finally, the adenine is converted to hypoxanthine, by adenine deaminase, which in turn is converted to urate and hydrogen peroxide (H2O2). The rate of production of H2O2 is measured with the colorimetric reagent, 3,5-dichloro-2-hydroxybenzenesulfonic acid, by an increase in absorbance at 500-520 nm. The assay is supplied with AdoHcy as a positive control. The assay can be used with any purified SAM-dependent methyltransferase.Assay Range:The detection range of this assay is from 0.013-0.133umol/min/ml of methyltransferase activity which is equivalent to an absorbance increase of 0.01 to 0.1 per minute.Kit Components:S0070-84A: SAM Methyltransfrase Assay Buffer, 1x20mlS0070-84B: Adenosylhomocysteine, Positive Control 1mM, 1x200ul**S0070-84C: Methyltransferase Enzyme Mix, 5x300ulS0070-84D: SAM Methyltransferase Assay Buffer Additive, 1x200ulS0070-84E: SAM Colorimetric Mix, 1x1 vialS0070-84F: S-Adenosylmethionine, 3x1vial*S0070-84G: Microtiter plate, 1x96 wells**S0070-84H: HCl Assay reagent (20mM) 1x1mlStorage and Stability: Store *S0070-84G at RT. Store **S0070-84C and **S0070-84H at -20°C. Store other components at -70°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对SAM Methyltransferase Assay Kit, Colorimetric , BioAssay 该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到SAM Methyltransferase Assay Kit, Colorimetric , BioAssay 产品后将其置于RT/-20°C/-70°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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