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中文名称

Rubella IgG Antibodies, BioAssay ELISA Kit (German Measles)

英文名字
Rubella IgG Antibodies, BioAssay ELISA Kit (German Measles)
供应商
USBiological
产品货号
R9700-19J
产品报价
¥1/96Tests
产品说明书
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背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
Sample Type:SerumIntroduction:Rubella IgG ELISA is intended for use in evaluating serologic status to the rubella virus infection. It is also used to evaluate paired sera for the presence of a significant increase in specific Rubella IgG as indication of a recent or current rubella virus infection.Introduction:Rubella is a herpes virus. Generally rubella is considered a mild adolescent disease. However, a maternal infection can be transmitted through the placenta to the fetus, causing congenital rubella. Congenital rubella may result in chronic cardiac disease, growth retardation hepatosplenomegaly, malformations and other severe anomalies. Children born asymptomatic may develop these abnormalities later in life. To reduce the risk of such severe complications, accurate serological methods must be performed to determine the serologic status of childbearing-aged women. The presence of rubella-specific IgG in the bloodstream attests immunity to rubella. A woman tested to be non-immune can be educated on infection and differentiate rubella from other exanthematous diseases. Expecting women with current rubella infection should be counseled on the consequences of congenital infection.Test Principle:Purified Rubella antigen is coated on the surface of microwells. Diluted serum is added to the wells, and the Rubella IgG-specific antibody, if present, binds to the antigen during incubation. After washing the wells to remove unbound sample, antibody to human IgG conjugated with horseradish peroxidase (HRP) is added and incubated at 37°C for 30 minutes. Unbound conjugate is removed by a subsequent washing step. A solution of TMB Reagent is then added to the microwells. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgG-specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrators and controls.Kit Components:Antigen-Coated Wells (1 plate, 96 wells, 12x8 strips), Microtiter Wells coated with purified Rubella antigenEnzyme Conjugate Reagent (12ml), Contains goat anti-human IgG conjugated to horseradish peroxidase with preservativesSample Diluent (22ml), Contains phosphate buffer with preservativesNegative Calibrator: 0 IU/ml (150ul/vial), Contains Rubella IgG negative serum with preservativesCut-off Calibrator: 15 IU/ml (150ul/vial), Contains Rubella IgG positive serum with preservativesPositive Calibrator: 30 IU/ml (150ul/vial), Contains Rubella IgG positive serum with preservativesPositive Calibrator: 100 IU/ml (150ul/vial), Contains Rubella IgG positive serum with preservativesNegative Control (150ul/vial), Contains Rubella IgG negative serum with preservatives; Range stated on the label.Positive Control (150ul/vial), Contains Rubella IgG positive serum with preservatives; Range stated on the label.Wash Buffer Concentrate (20x) (50ml), Contains phosphate buffer and Tween 20TMB Reagent (11ml), Contains 3, 3’, 5, 5’ tetramethylbenzidine (TMB) stabilized in buffer solutionStop Solution -1N HCl (11ml), Diluted hydrochloric acidStorage and Stability:Store all components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
产品特点
针对Rubella IgG Antibodies, BioAssay ELISA Kit (German Measles)该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到Rubella IgG Antibodies, BioAssay ELISA Kit (German Measles)产品后将其置于4°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
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