- 中文名称
Resistin, Mouse BioAssay ELISA Kit
- 英文名字
- Resistin, Mouse BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- R1585-01K
- 产品报价
- ¥1/1Kit

- 产品说明书
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- 产品新闻

- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
- 产品描述
- This kit is a biotin-labeled-antibody-based sandwich enzyme immunoassay for the quantitative measurement of mouse resistin in serum and tissue culture medium. It is intended for in vitro and research use only.In the Mouse Resistin ELISA kit, calibrators or samples are incubated with a goat polyclonal anti-mouse resistin antibody coated in microtiter wells. The wells are washed and biotin-labeled polyclonal anti-mouse resistin antibody is added and incubated with captured resistin. After a thorough wash, streptavidin-horseradish peroxidase conjugate is added. After the subsequent incubation and washing steps, the conjugate bound is allowed to react with the substrate H2O2-tetramethylbenzidine. The reaction is stopped by the addition of acidic solution and the absorbance of the resulting yellow product is measured at 450nm. The absorbance is proportional to the concentration of resistin. A standard curve is constructed by plotting the absorbance values versus the resistin concentrations of the calibrators, and the concentrations of unknown samples are determined using this standard curve.Kit Components:R1585-01K1: Microtiter Plate, 1x96 wells R1585-01K2: Pab (Biotin) 1x13ml R1585-01K3: Streptavidin (HRP), 1x13mlR1585-01K4: Standard, 100ng/ml, 1x50ulR1585-01K5: Standard, 50ng/ml, 1x50ul R1585-01K6: Standard, 20ng/ml, 1x50ulR1585-01K7: Standard, 10ng/ml, 1x50ulR1585-01K8: Standard, 5ng/ml, 1x50ulR1585-01K9: Standard, 2ng/ml, 1x50ulR1585-01K10: Standard, 1ng/ml, 1x50ulR1585-01K11: Quality Control, (18-27ng/ml), 1x1vialR1585-01K12: Dilution Buffer (5X), 1x22mlR1585-01K13: Wash Buffer (10X), 1x100mlR1585-01K14: Substrate Solution (TMB), 1x13mlR1585-01K15: Stop Solution, H2SO4, 1x13mlStorage and Stability: Store standards *R1585-01K4-R1585-01K10 at 4°C; when diluted, aliquot and store at -20°C. Store R1585-01K11 powder at 4°C, liquid at -20°C. Store other components at 4°C. Stable for at least 3 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Resistin, Mouse BioAssay ELISA Kit该产品的特点优势,欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Resistin, Mouse BioAssay ELISA Kit产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
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- 注意
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