- 中文名称
Resistin, Human, BioAssay ELISA Kit
- 英文名字
- Resistin, Human, BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- R1585-01
- 产品报价
- ￥1/1Kit
- 产品说明书
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- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Human Resistin ELISA is a sandwich enzyme immunoassay for the quantitative measurement of human Resistin in serum, plasma and tissue culture medium.Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins which is termed the RELM family. It is also described as ADSF (Adipose Tissue-Specific Secretory Factor) and FIZZ3 (Found in Inflammatory Zone). Human resistin contains 108aa as a prepeptide. Its hydrophobic signal peptide is cleaved before its secretion. Resistin circulates in human blood as a dimeric protein consisting of two 92aa polypeptides, which are disulfide-linked via Cys26. Resistin may be an important link between obesity and insulin resistance. Mouse resistin, specifically produced and secreted by adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity to insulin. Steppan, et al., have suggested that resistin suppresses the ability of insulin to stimulate glucose uptake. They have also suggested that resistin is present at elevated levels in blood of obese mice. It is down regulated by fasting and antidiabetic drugs. Way, et al., on the other hand, have found that resistin expression is severly suppressed in obesity. It is stimulated by several antidiabetic drugs. Other studies have shown that mouse resistin increases during the differentiation of adipocytes. It also seems to inhibit adipogenesis. In contrast, the human adipogenic differentiation is likely to be associated with a down regulation of resistin gene expression. Principle: Standards or samples are incubated with a rabbit polyclonal anti-human resistin antibody coated in microtiter wells. After one-hour incubation and a washing, biotin-labeled polyclonal anti-human resistin antibody is added and incubated with captured resistin. After a thorough wash, streptavidin-horseradish peroxidase conjugate is added. After one hour incubation and the last washing step, the remaining conjugate is allowed to react with the substrate H2O2-tetramethylbenzidine. The reaction is stopped by addition of acidic solution and absorbance of the resulting yellow product is measured at 450nm. The absorbance is proportional to the concentration of resistin. A standard curve is constructed by plotting absorbance values versus resistin concentrations of standards. Concentrations of unknown samples are determined using this standard curve.Kit Components:R1585-01A: Microtiter Plate, 1x96wellsR1585-01B: Standard, 1x1vialR1585-01C1: High Control, 1x1vialR1585-01C2: Low Control, 1x1vialR1585-01D: Pab (Biotin), 1x13mlR1585-01E: Streptavidin (HRP), 1x13mlR1585-01F: Dilution Buffer, 1x10mlR1585-01G: Wash Solution (10X), 1x100mlR1585-01H: Substrate Solution (TMB), 1x13mlR1585-01J: Stop Solution (H2SO4), 1x13mlStorage and Stability: Store all components at 4ºC. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Resistin, Human, BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Resistin, Human, BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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