IH, Frozen; IC; Immunohistochemistry (IHC), Immunocytochemistry (ICC) and Western Blotting (WB). A concentration of 0.5-1.0 µg/mL is recommended for WB. Human TNFAIP1 has a predicted length of 316 residues and MW of 36 kDa. A concentration of 0.25-0.5 µg/mL is recommended for IHC to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. A concentration of 0.25-0.5 µg/mL is recommended for IHC to detect the protein in frozen tissues. A concentration of 0.25-0.5 µg/mL is recommended for ICC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A synthetic peptide (NKYVQLNVGGSLYYTTVRALTRHDTM) corresponding to a region (27-52 aa) from the N-terminus of human tumor necrosis factor, alpha-induced protein 1, endothelial (TNFAIP1).
来源宿主
兔
反应性
人; 大鼠; predicted to react with 小鼠 due to sequence homology;