WB; IH,Frozen; IH(P), Paraffin Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0 µg/mL is recommended for WB. Human Cytokeratin 8 has a predicted length of 483 amino acids and MW of 54 kDa. A concentration of 2.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A synthetic peptide (QQQKTARSNMDNMFESYIN) corresponding to a region (127-145) from human Cytokeratin 8. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
