WB; IH,Frozen; IH(P), Paraffin Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human MIF has a predicted length of 115 amino acids and MW of 12 kDa. A concentration of 0.5-1 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A synthetic peptide (NYYDMNAANVGWNNSTFA) corresponding to a region (98-115) from the C-terminus of human macrophage migration inhibitory factor (MIF).
来源宿主
兔
反应性
人; 大鼠; predicted to react with 小鼠 due to sequence homology