IHC, Frozen; IH(P), Paraffin embedded; IF, Frozen; IF(P), Paraffin embedded; IC; ELISA Immunohistochemistry/cytochemistry on 4% PFA or formalin fixed frozen sections; paraffin sections can be more difficult and require more extensive antigen recovery methods. 1:400-1:2000 depending up detection and incubation times ELISA: direct antigen ELISA This antiserum will superbly stain both cell bodies and nerve terminal, and works particularly well in enteric and peripheral neurons. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A synthetic peptide (GLFSSYRLPGHTQDTLVAQKSS) as a part of porcine ChAT protein (aa: 167-188) conjugated to KLH
来源宿主
兔
反应性
This antiserum is known to react with ChAT of origin guinea-猪, 小鼠, 大鼠, 兔.