Raf 1 抗体-Raf 1 Antibody代理/参数/厂家-一抗-艾美捷科技有限公司

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背景资料: A-Raf, B-Raf and c-Raf (Raf-1) are the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway (1). Activation of c-Raf is the best understood and involves phosphorylation at multiple activating sites including Ser338, Tyr341, Thr491, Ser494, Ser497 and Ser499 (2). p21-activated protein kinase (PAK) has been shown to phosphorylate c-Raf at Ser338 and the Src family phosphorylates Tyr341 to induce c-Raf activity (3, 4). Ser338 of c-Raf corresponds to similar sites in A-Raf (Ser299) and B-Raf (Ser445), although this site is constitutively phosphorylated in B-Raf (5). Inhibitory 14-3-3 binding sites on c-Raf (Ser259 and Ser621) can be phosphorylated by Akt and AMPK, respectively (6, 7). While A-Raf, B-Raf and c-Raf are similar in sequence and function, differential regulation has been observed (8). Of particular interest, B-Raf contains three consensus Akt phosphorylation sites (Ser364, Ser428 and Thr439) and lacks a site equivalent to Tyr341 of c-Raf (8, 9). The B-Raf mutation V600E results in elevated kinase activity and is commonly found in malignant melanoma (10). Six residues of c-Raf (Ser29, Ser43, Ser289, Ser296, Ser301 and Ser642) become hyperphosphorylated in a manner consistent with c-Raf inactivation. The hyperphosphorylation of these six sites is dependent on downstream MEK signaling and renders c-Raf unresponsive to

免疫原: Raf-1 antibody was raised against a peptide sequence around aa. 641-645 (T-S-P-R-L ) derived from Rat Raf-1.

其他: ProSci Incorporated于1998年成立于美国加州圣地亚哥市,从事抗体等科研试剂的研发、生产和销售。产品涵盖了细胞凋亡、信号转导、免疫、肿瘤、细胞生物、神经生物、传染病等领域。ProSci拥有超过30000种单克隆抗体和多克隆抗体。ProSci的抗体大都经免疫亲和层析纯化,具有高纯度、高亲和力、特异性强等特点,所有的ProSci抗体都经过了严格的验证测试和质量控制,已保证高质量的产品。此外,ProSci还提供标记二抗,重组蛋白,细胞和组织裂解液,细胞涂片,组织切片,多肽等产品与抗体配套使用。