中文名称

Wnt-9b 重组蛋白

英文名字

Wnt-9b Recombinant Protein

供应商

ProSci

产品货号

PSI-40-653

产品报价

￥询价/5ug

产品说明书

点击查看

购买方式

银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868

产品新闻

背景资料

Formerly known as Wnt-15 or Wnt-14b, Wnt-9b is a secreted glycoprotein belonging to the Wnt family of signaling proteins that are critically involved in maintaining the integrity of both embryonic and adult tissues. Wnt-9b is primarily expressed in adult kidneys and during late embryogenesis, and shares with other Wnt family members the same highly conserved lipid-modified, cysteine-rich domain essential for cell signaling. As is true for most Wnt family members, Wnt-9b functions through the biochemical process known as the canonical Wnt pathway. In this process, Wnt proteins bind to and activate seven-pass transmembrane receptors of the Frizzled family, and ultimately results in the disruption of β-catenin degradation. Intracellular accumulation of β-catenin increases translocation of the protein into the nucleus, where it binds to TCF/LEF transcription factors to promote the expression of numerous genes. In this manner, Wnt signaling induces and maintains transformed phenotype, and, in certain embryonic cell lines, supports self-renewal in the absence of significant differentiation. While increased Wnt/β-catenin signaling is associated with tumorigenesis in a diverse set of human cancers, lack of Wnt signaling disrupts transcriptional activation of tumor suppressor genes, and has been shown to result in neoplastic transformation, oncogenesis, and human degenerative diseases. Altered Wnt-9b expression has been shown to result in the underdevelopment of the kidneys, and incomplete lip and cleft fusion in mice. Recombinant Human Wnt-9b is a monomeric glycoprotein containing 335 amino acid residues and has a calculated molecular weight of 36.9 kDa. Due to glycosylation, Recombinant Human Wnt-9b migrates at an apparent molecular weight of approximately 49-54 kDa by SDS-PAGE analysis under non-reducing conditions.

序列

SYFGLTGREV LTPFPGLGTA AAPAQGGAHL KQCDLLKLSR RQKQLCRREP GLAETLRDAA HLGLLECQFQ FRHERWNCSL EGRMGLLKRG FKETAFLYAV SSAALTHTLA RACSAGRMER CTCDDSPGLE SRQAWQWGVC GDNLKYSTKF LSNFLGSKRG NKDLRARADA HNTHVGIKAV KSGLRTTCKC HGVSGSCAVR TCWKQLSPFR ETGQVLKLRY DSAVKVSSAT NEALGRLELW APARQGSLTK GLAPRSGDLV YMEDSPSFCR PSKYSPGTAG RVCSREASCS SLCCGRGYDT QSRLVAFSCH CQVQWCCYVE CQQCVQEELV YTCKH

来源宿主

CHO 细胞

溶解建议

艾美捷温馨提示:开盖前,请先离心。厂家建议您，按照该货号产品的说明书中要求的方式进行溶解和使用。

保存建议

The recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted protein is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing the recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

其他

ProSci Incorporated于1998年成立于美国加州圣地亚哥市,从事抗体等科研试剂的研发、生产和销售。产品涵盖了细胞凋亡、信号转导、免疫、肿瘤、细胞生物、神经生物、传染病等领域。ProSci拥有超过30000种单克隆抗体和多克隆抗体。ProSci的抗体大都经免疫亲和层析纯化,具有高纯度、高亲和力、特异性强等特点,所有的ProSci抗体都经过了严格的验证测试和质量控制,已保证高质量的产品。此外,ProSci还提供标记二抗,重组蛋白,细胞和组织裂解液,细胞涂片,组织切片,多肽等产品与抗体配套使用。

注意

该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。

分享到：