RAB34 protein which regulates a fast endocytic recycling pathway is restricted to the plasma membrane and endocytic compartments. RAB34 takes part in protein transport, specifically the redistribution of lysosomes to the peri-Golgi region. In fibroblasts, RAB34 protein was co-localized with actin to the membrane ruffles and membranes of moderately large vesicles close to the ruffles.