Mediator complex subunit 4 （MED4） is a component of the Mediator complex， a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED4 is a component of the vitamin D receptor-interacting protein （DRIP） complex which functions as a nuclear receptor coactivator. The DRIP complex is able to activate nuclear receptors in a ligand-dependent manner. MED4 functions as a link to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription apparatus. MED4 is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
序列
请咨询我们客服或者请查看品牌官方网站
来源宿主
大肠杆菌中重组表达
溶解建议
Sterile filtered colorless solution. The MED4 protein solution contains 20mM Tris-HCl buffer （pH8.0）， 20% glycerol， 1mM DTT and 100mM NaCl.
保存建议
Store at 4℃ if entire vial will be used within 2-4 weeks. Store， frozen at -20℃ for longer periods of time. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Avoid multiple freeze-thaw cycles.
