ISG15 ubiquitin-like Modifier is conjugated to the intracellular target proteins after IFN-alpha or IFN-beta stimulation. ISG15 enzymatic pathway is partially distinct from that of ubiquitin， differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme， but seems to converge with the ub conjugation pathway at the level of a specific E2 enzyme. ISG15 protein targets include STAT1， SERPINA 3G/SPI2A， JAK1， MAPK3/ERK1， PLCG1， EIF2AK2/PKR， MX1/MXA， and RIG-1. Deconjugated by USP18/UBP43 shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. ISG15 serves as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. ISG15 plays a role in autocrine， paracrine and endocrine mechanisms， as in cell-to-cell signaling， possibly partly by inducing ifn-gamma secretion by monocytes and macrophages. ISG15 displays antiviral activity during viral infections. ISG15 in response to IFN-TAU secreted by the conceptus， ligates to and regulate proteins involved in the release of prostaglandin F2-alpha and thus prevent lysis of the corpus luteum and maintain the pregnancy.
The interferon-stimulated gene having a molecular mass of 15 kDa is an interferon regulated gene that is induced as a primary response to diverse microbial and cell stress stimuli， and encodes the founding member of the ubiquitin-like protein family. ISG15 shares several common properties with other ubiquitin-like molecules， but its activity is strongly regulated by precise signaling pathways that have a role in innate immunity.
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来源宿主
大肠杆菌中重组表达
溶解建议
Sterile Filtered colorless solution. The ISG15 solution contains 20mM Tris-HCl pH-8， 100mM NaCl and 1mM DTT.
保存建议
ISG15 although stable 4℃ for 4 weeks， should be stored desiccated below -18℃.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles.
