High-mobility group box 1 protein （HMGB1）， previously known as HMG-1 or amphoterin， is a member of the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 30 kDa， 215 amino acid （aa） single chain polypeptide containing three domains: two N-terminal globular， 70 aa positively charged DNA-binding domains （HMG boxes A and B）， and a negatively charged 30 aa C-terminal region that contains only Asp and Glu.4， 5 Residues 27 - 43 and 178 - 184 contain a NLS. Posttranslational modifications of the molecule have been reported， with acetylation occurring on as many as 17 lysine residues. HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins.
序列
请咨询我们客服或者请查看品牌官方网站
来源宿主
大肠杆菌中重组表达
溶解建议
Sterile Filtered White lyophilized （freeze-dried） powder. The HMG1 （1 mg/ml） was lyophilized after extensive dialyses against 1x PBS pH-7.4. It is recommended to reconstitute the lyophilized HMGB1 in sterile 18MΩ-cm H2O not less than 100 ug/ml， which can then be further diluted to other aqueous solutions.
保存建议
Lyophilized HMGB1 although stable at room temperature for 3 weeks， should be stored desiccated below -18℃. Upon reconstitution HMGB1 should be stored at 4℃ between 2-7 days and for future use below -18℃.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles.
