GFP is an intermediate filament. GFP and vimentin are linked to the same filament network; they are localized in the same filaments.
mRNAs encoding the glial intermediate filament protein are spatially dispersed in the glial cell cytoplasm close to the location of the glial filaments.
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牛脊髓中提取纯化
溶解建议
Sterile Filtered White lyophilized (freeze-dried) powder. The protein was lyophilized from a 1mg/ml solution containing 10mM sodium phosphate buffer pH 7.5, 6M urea, 2mM DTT, 1mM EDTA and 10mM methylammonium chloride. It is recommended to reconstitute the lyophilized GFP in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
保存建议
Lyophilized GFP although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution GFP should be stored at 4℃ between 2-7 days and for future use below
-18℃.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.