Osteocrin is a recently identified secreted protein expression of which was only detected in bone， peaking just after birth and decreasing markedly with age. A 1280-bp mRNA encodes osteocrin producing a mature protein of 103 amino acids with a molecular mass of 11.4 kDa. In primary osteoblastic cell cultures osteocrin expression coincided with matrix formation then decreased in very mature cultures. Treatment of cultures with 1，25-dihydroxyvitamin D3 resulted in a rapid dose- dependent down-regulation of osteocrin expression， suggesting direct regulation. Chronic treatment of primary cultures with osteocrin-conditioned media inhibited mineralization and reduced osteocalcin and alkaline phosphatase expression. These results suggest that osteocrin represents a novel， unique vitamin D-regulated bone-specific protein that appears to act as a soluble osteoblast regulator.
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来源宿主
大肠杆菌中重组表达
溶解建议
Filtered White lyophilized （freeze-dried） powder. Filtered （0.4um） and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer， pH 4.0. It is recommended to add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value， we recommend intensive dilution by relevant buffer to a concentration of 10ug/ml. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
保存建议
Store lyophilized protein at -20℃. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4℃ for a limited period of time； it does not show any change after two weeks at 4℃. The lyophilized protein remains stable until the expiry date when stored at -20℃.
