Thioredoxins are small disulphide-containing redox proteins （within the conserved Cys-Gly-Pro-Cys active site） that have been found in all the kingdoms of living organisms. Thioredoxin contains a single disulfide active site and serves as a general protein disulphide oxidoreductase. Thioredoxins are involved in the first unique step in DNA synthesis. It interacts with a broad range of proteins by a redox mechanism based on reversible oxidation of two cysteine thiol groups to a disulphide， accompanied by the transfer of two electrons and two protons. The net result is the covalent interconversion of a disulphide and a dithiol. It has been suggested that thioredoxin may catalyze the formation of correct disulfides during protein folding because of its ability to act as an efficient oxidoreductant. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation.
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来源宿主
大肠杆菌中重组表达
溶解建议
Sterile Lyophilized Powder. Each mg of protein contains 20mM phosphate buffer pH 7.4. It is recommended to reconstitute the lyophilized TRX in sterile 18MΩ-cm H2O.
保存建议
TRX although stable at 4℃ for 3 weeks， should be stored desiccated below -18℃.
Please prevent freeze thaw cycles.
