Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Binds to and activates cyclin-dependent kinase CDK8 that phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex.Lew D.J., Cell 66:1197-1206(1991).Li H., Genomics 32:253-259(1996).Mungall A.J., Nature 425:805-811(2003).
应用类型
WB
免疫原
Synthesized non-phosphopeptide derived from human Cyclin C around the phosphorylation site of serine 275 (N-G-S(p)-Q-N).
