Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage.Saunders W.S., J. Cell Sci. 104:573-582(1993).Ye Q., J. Biol. Chem. 271:14653-14656(1996).Ye Q., J. Biol. Chem. 272:14983-14989(1997).
应用类型
WB,IHC
免疫原
Synthesized non-phosphopeptide derived from human APLF around the phosphorylation site of serine 116 (R-N-S(p)-Q-V).
