Flavokinase is a transferases family member， specifically those transferring phosphorus-containing groups （phosphotransferases） with an alcohol group as acceptor. Flavokinase is an enzyme that catalyzes the phosphorylation of riboflavin （vitamin B2） to form flavin-mononucleotide （FMN）， which is an obligatory step in vitamin B2 utilization and flavin cofactor synthesis. It has been proposed that TNF， through the activation of the RFK gene， enhances the incorporation of FAD in NADPH oxidase enzymes， which is a critical step for the assembly and activation of NADPH oxidase.
