The Profilin phospho-regulation antibody sampler kit can be used to detect phosphorylation of Tyr-129 and Ser-138 relative to total profilin expression levels. The kit includes rabbit polyclonal phospho-specific antibodies to Tyr-129 and Ser-138, as well as rabbit polyclonal antibodies that detect different epitopes in the C-terminal region of Profilin. The kit also includes an anti-Rabbit Light Chain specific:HRP secondary reagent for detection of rabbit polyclonal antibody in western blot, ELISA, or immunocytochemistry.