- 中文名称
Protein Kinase C Assay Kit, BioAssay (PKC) discontinued
- 英文名字
- Protein Kinase C Assay Kit, BioAssay (PKC) discontinued
- 供应商
- USBiological
- 产品货号
- P9105-98
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The assay kit is designed to measure the phosphotransferase activity of protein kinase C (PKC) in immunoprecipitates and column fractions. It is highly specific for alpha, beta and gamma. Crude cell lysates may also be used but detergents/biochemicals contained in the cell lysis buffer may inhibit PKC kinase activity. Furthermore, although two inhibitors are included with the kit, editors may suggest other unknown kinases found in crude lysates are responsible for substrate peptide phosphorylation. The assay kit is based on phosphorylation of a specific substrate peptide (QKRPSQRSKYL) using the transfer of the -phosphate of adenosine-5'-[32P] triphosphate ([ -32P] ATP) by PKC kinase. The phosphorylated substrate is then separated from the residual [ -32P] ATP using P81 phosphocellulose paper and quantitated by using a scintillation counter. The assay is linear for incubation times of up to 30 minutes and incorporation of up to 20% of total ATP. Further incubation or incorporation may not be linear and may therefore not be a true indication of PKC activity in the sample extract. The enzyme assay is rapid, convenient and fairly specific for PKC. Capacity:~200 PKC kinase assays. Kit Components: 1. Assay Dilution Buffer II (ASBII) containing 20mM MOPS, pH 7.2, 25mM β-glycerol phosphate, 1mM sodium orthovanadate, 1mM dithiothreitol, 1mM CaCl , 2x1ml2. PKC Substrate Cocktail, 500uM PKC substrate peptide [QKRPSQRSKYL] in ADBII, 2x1ml3. PKA/CaMK Inhibitor Cocktail containing 2uM PKA inhibitor peptide, and 20uM Compound R24571 in ADBII, 2x1ml4. PKC Lipid Activator containing 0.5mg/ml phosphatidyl serine and 0.05mg/ml diacylglycerol in ADBII, 2x1ml5. Mg/ATP Cocktail containing 75mM magnesium chloride and 500um ATP 20mM MOPS, pH 7.2, 25mM β-glycerophosphate, 5mM EGT A, 1mM Na3VO4, 1mM dithiothreitol, 2x1ml. (Before starting the assay, 90ul of the Mg2+/ATP cocktail should be added to 100uCi (10ul) of the [γ-32P]ATP (∼3000Ci/mmol).)6. p81 squares, 1x200 eachStorage and Stability:Store components at -20°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Other components required but not included as part of kit are: Sample preparation containing Protein Kinase C: 10-200ug protein/immunoprecipitate diluted into assay dilution buffer or 25-100ng of purified Protein Kinase C vortex mixer plexiglass shielding 30°C incubating water bath timer variable volume (5-200ul) pipet + tips 0.75% phosphoric acid scintillation vials scintillation fluid scintillation counter [gamma-32P]ATP-3000 Ci/mmol,
- 产品特点
- 针对Protein Kinase C Assay Kit, BioAssay (PKC) discontinued该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Protein Kinase C Assay Kit, BioAssay (PKC) discontinued产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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