- 中文名称
Protein Assay Kit, BioAssay
- 英文名字
- Protein Assay Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- P9102-20P
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The protein is known as the building blocks of life and is one of the most important macromolecules in life science. Proteins are polypeptides made up of amino acids and play various key roles in all aspects of biology. Protein quantitation is a very common practice for life scientists.Simple, direct and automation-ready procedures for measuring protein concentration are very desirable. Protein assay kit is based on an improved Coomassie Blue G method. The dye forms a blue complex specifically with protein, and the intensity of color, measured at 595nm, is directly proportional to the protein concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples and exhibits increased sensitivity towards peptides.Applications:Direct Assays: total protein concentration.Key Features:Sensitive and accurate. Use 10ul samples. Detection range 0.06–1.0mg /mL protein in 96-well plate assay.Simple and high-throughput. The “mix-and-read” procedure involves addition of a single working reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.Low interference. Glucose, Tris, vitamins, and amino acids, DNA, RNA, salts, EDTA (< 12mM), phenol (< 50mM), urea (< 0.6 M), Triton (< 0.1%) and SDS (< 0.1% SDS) do not interfere in the assay.Versatility: assays can be executed in 96-well plate or cuvet.Kit Contents: (500 tests in 96-well plates)Reagent: 20ml 5 x concentrateProtein standard: 1ml 1.0 mg/mL BSAStorage conditions. The kit is shipped at room temperature. Store the reagent at 4°C and standard at -20°C, respectively. Shelf life: 12 months after receipt.Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.Procedures:Reagent Preparation:Prepare enough working reagent by adding 1 vol of the 5 x Reagent to 5 vol of distilled water. Bring reagent to room temperature before use.Procedure using 96-well plate:1. Dilute standard as shown in the Table. Transfer 10ul diluted Standards and diluted sample in duplicate wells of a clear bottom 96-well plate. Store diluted standards at -20°C for future use.No Premix+H2O Vol (ul) BSA (mg/mL) BSA (ug/10ul)1 100ul+0ul 100 1.0 102 80ul+20ul 100 0.8 83 60ul+40ul 100 0.6 64 40ul+60ul 100 0.4 45 30ul+70ul 100 0.3 36 20ul+80ul 100 0.2 27 10ul+90ul 100 0.1 18 0ul+100ul 100 0 02. Add 200ul working reagent and tap lightly to mix.3. Measure OD at 570-630nm (peak 595nm).Procedure using cuvette:1. Prepare standards as in the 96-well plate assay. Transfer 50ul diluted Standards and 50ul samples to cuvets.2. Add 1000ul working reagent and tap lightly to mix.3. Measure OD at 570-630nm (peak 595nm).Calculation:Subtract blank OD (water, #8) from the standard OD values and plot the OD against standard concentrations. Use the standard curve to determine the sample protein concentration.Materials Required, But Not Provided:Pipeting devices and accessories.Procedure using 96-well plate:Blank 96-well plates (e.g. Corning Costar).Plate reader for 96-well plate.Procedure using cuvette:Cuvets and spectrophotometer.General Considerations:If protein concentration is > 1 mg/mL, dilute samples in distilled water, and use OD values that lie within the calibration curve to calculate the sample protein concentration. Reading can be performed as soon as the reagent and sample are mixed. High sensitivity can be achieved by adding 50ul sample to 200ul Reagent (detection range 3–200 ug/mL).
- 产品特点
- 针对Protein Assay Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Protein Assay Kit, BioAssay产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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