- 中文名称
Progesterone, Human, BioAssay ELISA Kit
- 英文名字
- Progesterone, Human, BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- P9006-04H
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Sample Type:Serum/PlasmaIntended Use:For the quantitative determination of Progesterone concentration in human serum or plasmaIntroduction:Progesterone is a C21 steroid which is synthesized from both tissue and circulating cholesterol. Cholesterol is transformed to pregnenolone which is then converted via a combined dehydrogenase and isomerase to progesterone. The principle production sites are the adrenals and ovaries and the placenta during pregnancy. The majority of this steroid is metabolized in the liver to pregnanediol and conjugated as a glucuronide prior to excretion by the kidneys. Progesterone exhibits a wide variety of end organ effects. The primary role of progesterone is exhibited by the reproductive organs. In males, progesterone is a necessary intermediate for the production of corticosteroids and androgens. In females, progesterone remains relatively constant throughout the follicular phase of the menstrual cycle. The concentration then increases rapidly following ovulation and remains elevated for 4-6 days and decreases to the initial level24 hours before the onset of menstruation. In pregnancy, placental progesterone production rises steadily to levels of 10 to 20 times those of the luteal phase peak. Progesterone measurements are thus performed to determine ovulation as well as to characterize luteal phase defects. Monitoring of progesterone therapy and early stage pregnancy evaluations comprise the remaining uses of progesterone assays. Progesterone EIA kits are designed for the measurement of total progesterone in human serum or plasma.Test Principle:Progesterone EIA is based on the principle of competitive binding between progesterone in the test specimen and progesterone-HRP conjugate for a constant amount of rabbit antiprogesterone. In the incubation, goat anti-rabbit IgG-coated wells are incubated with 25ul progesterone standards, controls, patient samples, 100ul progesterone-HRP Conjugate Reagent and 50ul rabbit anti-progesterone reagent at room temperature (18-25°C) for 90 minutes. During the incubation, a fixed amount of HRP-labeled progesterone competes with the endogenous progesterone in the standard, sample, or quality control serum for a fixed number of binding sites of the specific progesterone antibody. Thus, the amount of progesterone peroxidase conjugate immunologically bound to the well progressively decreases as the concentration of progesterone in the specimen increases. Unbound progesterone peroxidase conjugate is then removed and the wells washed. Next, a solution of TMB Reagent is then added and incubated at room temperature for 20 minutes, resulting in the development of blue color. The color development is stopped with the addition of Stop Solution, and the absorbance is measured spectrophotometrically at 450 nm. The intensity of the color formedis proportional to the amount of enzyme present and is inversely related to the amount of unlabeled progesterone in the sample. A standard curve is obtained by plotting the concentration of the standard versus the absorbance. The progesterone concentration ofthe specimens and controls run concurrently with the standards can be calculated from the standard curve.Kit Components:Goat Anti-Rabbit IgG-coated microtiter wells, 96 wells.Standard, 0g/ml, 1x500ulStandard, 0.5ng/ml, 1x500ulStandard, 3ng/ml, 1x500ulStandard, 10ng/ml, 1x500ulStandard, 25ng/ml, 1x500ulStandard, 50ng/ml, 1x500ulRabbit Anti-Progesterone Reagent (pink color), 7ml.Progesterone-HRP Conjugate Concentrate (11x), 1.3ml.Progesterone-HRP Conjugate Diluent, 13mlProgesterone Control 1, Liquid, 0.5ml, Ready to use.Progesterone Control 2, Liquid, 0.5ml, Ready to use.TMB Reagent (One-Step), 11ml.Stop Solution (1N HCl), 11ml.Storage and Stability:Store all components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Progesterone, Human, BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Progesterone, Human, BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
-
- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
-
