Plasma phospholipid transfer protein (PLTP) is thought to play a major role in the facilitated transfer of phospholipids between lipoproteins and in the modulation of highdensity lipoprotein (HDL) particle size and composition. PLTP-facilitated lipid transfer activity is related to HDL and LDL metabolism, as well as lipoprotein lipase activity, adiposity, and insulin resistance. The PLTP Drug Screening Kit utilizes rabbit serum as PLTP activity source to screen PLTP inhibitors. The assay uses a donor molecule containing a fluorescent self-quenched phospholipid that is transferred to an acceptor molecule in the presence of PLTP. PLTP-mediated transfer of the fluorescent phospholipid to the acceptor molecule results in an increase in fluorescence (Excitation: 465nm; Emission: 535nm). Inhibitor of PLTP will inhibit the lipid transfer and therefore decrease fluorescence intensity. The kit provides sufficient reagents for 100 PLTP inhibitor screening assays.Kit Components:PLTP Donor Molecule 1x1mlPLTP Acceptor Molecule 1x1mlPLTP Assay Buffer (10X) 1x5mlPositive Control (Rabbit Serum) 1x0.3ml.Storage and Stability:Store the kit at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
产品特点
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保存建议
建议收到PLTP Inhibitor Drug Screening BioAssay Kit产品后将其置于4°C保存。
