- 中文名称
Plasminogen Activator (tPA) BioAssay ELISA Kit (Antibodies only)
- 英文名字
- Plasminogen Activator (tPA) BioAssay ELISA Kit (Antibodies only)
- 供应商
- USBiological
- 产品货号
- P4256-33
- 产品报价
- ￥1/1Kit
- 产品说明书
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- 购买方式
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Tissue-type plasminogen activator (tPA) is one of two major physiologic activators of plasminogen in plasma. It is a serine protease of 68 kDa produced primarily in endothelial cells but is also present in monocytes and megakaryocytes. Normal plasma tPA antigen concentrations have been reported from 20ng/ml to 5ug/ml, depending on the assay used, but typically most of the tPA (>90%) is in complex with it’s primary inhibitor, plasminogen activator inhibitor-1 (PAI-1). Structurally, tPA is a single-chain enzyme that consists of a catalytic domain followed by two kringle structures, an EGF domain and a finger domain. The activation of plasminogen by tPA is dependent on the presence of a fibrin cofactor. The binding of both tPA and plasminogen to fibrin is mediated in part through lysine binding sites within the kringle structures of both enzyme and substrate, but also through the finger domain of tPA. Activation of plasminogen by tPA occurs by cleavage after residue Arg560 to produce the two-chain active serine protease plasmin. The activity of tPA is regulated in part by a very short half life in circulation (t1⁄2 of ~4 minutes) and by circulating protease inhibitors PAI-1 and to a lesser extent alpha2macroglobulin.Principle:Affinity-purified antibody to tPA is coated onto the wells of a microtitre plate. Any remaining binding sites on the plastic wells are blocked with an excess of bovine serum albumin. The plates are washed and plasma or other fluids containing tPA are applied. The coated antibody will capture the tPA in the sample. After washing the plate to remove unbound material, a peroxidase conjugated second antibody to tPA is added to the plate to bind to the captured tPA. After washing the plate to remove unbound conjugated antibody, the peroxidase activity is expressed by incubation with o-phenylenediamine (OPD). After a fixed development time the reaction is quenched with the addition of H2SO4 and the color produced is quantified using a microplate reader. The color generated is proportional to the concentration of tPA present in the sample.Quantity: Sufficient reagents for 5x96 well platesKit Components:P4256-33A: Capture antibody, 1x500ul (Yellow cap). Polyclonal affinity purified anti-tPA, 50% glycerol. Suitable for use as coating antibody P4256-33B: Detection antibody 1x500ul (Red cap). Polyclonal affinity purified anti-tPA, 50% glycerol. Suitable for use as detection antibodyStorage and Stability:Store components at -20°C. Stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Plasminogen Activator (tPA) BioAssay ELISA Kit (Antibodies only)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Plasminogen Activator (tPA) BioAssay ELISA Kit (Antibodies only)产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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