- 中文名称
PEP, Colorimetric/Fluorometric Assay Kit, BioAssay (Phosphoenolpyruvate)
- 英文名字
- PEP, Colorimetric/Fluorometric Assay Kit, BioAssay (Phosphoenolpyruvate)
- 供应商
- USBiological
- 产品货号
- P4072-54M
- 产品报价
- ¥1/100Tests

- 产品说明书
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- 购买方式
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- 产品新闻

- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
- 产品描述
- Phosphoenolpyruvate (PEP) is an important intermediate in carbohydrate metabolism. Containing a high-energy phosphate bond, PEP is involved in glycolysis and gluco-neogenesis and in the shikimate pathway as well as in carbon fixation in plants. Bacteria utilize PEP in the phosphor-transferase system to acquire sugars from the environment. In the glycolytic pathway, PEP is formed from 2-phosphoglycerate by enolase and generates ATP through the action of pyruvate kinase. USBio’s PEP BioAssay Kit provides a convenient colorimetric and fluorometric means to measure PEP levels in various samples. In the assay, PEP is converted to ATP and pyruvate. The generated pyruvate is quantified by colorimetric (lmax = 570nm) or fluorometric methods (Ex/Em = 535/587nm). The assay is simple, sensitive and reliable. The detection limit is approximately 1uM PEP in biological samples.Sample Type:Cell/tissue lysates, urine, plasma, serum, and plant extracts.Intended Use:A convenient colorimetric and fluorometric means to measure PEP levels in various samples.Sensitivity:1uMSpecificity:MammalianTest Principle:In the assay, PEP is converted to ATP and pyruvate. The generated pyruvate is quantified by colorimetric (lmax = 570nm) or fluorometric methods (Ex/Em = 535/587nm).Kit Components:PEP Assay Buffer: 1x25mlPEP Probe (in DMSO): 1x200ulPEP Converter: 1 vialPEP Developer Mix: 1 vialPEP Standard (1umole): 1 vialStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对PEP, Colorimetric/Fluorometric Assay Kit, BioAssay (Phosphoenolpyruvate)该产品的特点优势,欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到PEP, Colorimetric/Fluorometric Assay Kit, BioAssay (Phosphoenolpyruvate)产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译,仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前,请浏览说明书确认。
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