Phosphatidylcholine (PC) is a phospholipid which incorporates choline as the headgroup of the lipid. PC is a major constituent of biological membranes and is involved in cell signaling through release of choline by phospholipase D leaving the second messenger phosphatidic acid. This Phosphatidylcholine BioAssay Kit is a simple convenient means of measuring Phosphatidylcholine in a variety of biological samples. The kit utilizes an enzyme-coupled assay in which PC is hydrolyzed, releasing choline which is subsequently oxidized resulting in development of the OxiRed probe to generate fluorescence (Ex/Em 535nm / 587nm) and absorbance (570 nm). Phosphatidylcholine kit measures PC in the range of 0.1-10nmol per sample. PC is present in serum at ~ 0.2-2.5mM (~50-200mg/dL)Kit Components:1. PC Assay Buffer, 1x25ml2. OxiRed Probe, 1x200ul3. PC Hydrolysis Enzyme, 1x1vial4. PC Development Mix, 1x1vial5. PC Standard (10umol), 1x1vialStorage and Stability:Store components at -20°C, protect from light. Stable for 3 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
