Phosphate is one of the most important of the inorganic ions in biological systems. It functions in a variety of roles. One of the most important roles is as a molecular switch, turning enzyme activity on and off through the mediation of the various protein kinases and phosphatases in biological systems. Phosphate is also of great importance in mineralization processes and is a primary stimulus of algal blooms frequently found in bodies of fresh water, due to run‐off from areas of high fertilizer use. The newly designed Phosphate Colorimetric Assay Kit provides an easy, quick and sensitive means of assessing phosphate over a wide range of concentrations. The assay utilizes a proprietary formulation of malachite green and ammonium molybdate which forms a chromogenic complex with phosphate ion giving an intense absorption band around 650nm. Phosphate concentrations between 1uM and 1mM, with a lower limit of detectionof ~0.1nmol, can be directly determined. The Phosphate Colorimetric Assay Kit provides 500assays using microtiter plates or 100assays using 1ml cuvettes.Kit Components:Phosphate Reagent 1x15mlPhosphate Standard (10mM) 1x0.5mlStorage and Stability:Store the kit at RT. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
