- 中文名称
PRDX1, Human, BioAssay ELISA Kit (Peroxiredoxin-1, Thioredoxin Peroxidase 2, Thioredoxin-dependent Peroxide Reductase 2, Proliferation-associated Gene Protein, PAG, Natural Killer Cell-enhancing Facto
- 英文名字
- PRDX1, Human, BioAssay ELISA Kit (Peroxiredoxin-1, Thioredoxin Peroxidase 2, Thioredoxin-dependent Peroxide Reductase 2, Proliferation-associated Gene Protein, PAG, Natural Killer Cell-enhancing Factor A, NKEF-A, TDPX2, PAGB, PAGA)
- 供应商
- USBiological
- 产品货号
- P3351-95P
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Organisms living under aerobic conditions have developed various anti-oxidative mechanisms to protect them from damage by reactive oxygen species (ROS). Peroxiredoxin (Prx) is a growing anti-oxidative protein family that has been identified six members in mammals. They share a common reactive Cys residue in the N-terminal region, and are capable of serving as a peroxidase and involve thioredoxin and/or glutathione as the electron donor. Prx1 to Prx4 have an additional Cys residue in the conserved C-terminal region, and are cross members as judged by the aa sequence similarity. Prx5 also contains an additional Cys in its C-terminal region that is less conserved. On the other hand, Prx6 has only one unique Cys. These Prx family members are distributed in subcellular localization, Prx1, 2, and 6 in cytosol, Prx3 in mitochondria, Prx4 in ER and secretion, Prx5 showing complicated distribution including peroxisome, mitochondria and cytosol, all of which are potential sites of ROS production. In addition to their role as a peroxidase, however, a body of evidence has accumulated to suggest that individual members also serve divergent functions, which are associated with various biological processes such as the detoxification of oxidants, cell proliferation, differentiation and gene expression. It would be expected that these functions might not necessarily depend on peroxidase activity and, therefore, it seems likely that the divergence is due to unique molecular characteristics intrinsic to each member.Intended Use:Human Peroxiredoxin-1 (human Prx1) ELISA kit is to be used for the in vitro quantitative determination of human Prx1 in human serum, human plasma, cell lysate and buffered solution. The assay will recognize both native and recombinant human Prx1. This kit has been configured for research use only and is not to be used in diagnostic procedures.Sensitivity: The minimal detectable dose of human Prx1 was calculated to be 0.5ng/ml, by subtracting two standard deviations from the mean of 10 zero standard replicates (ELISA buffer, S0) and intersecting this value with the standard curve obtained in the same calculation.Specificity:The following substances were tested and found to have no cross-reactivity: human Prx2, Prx3, prx4, Prx5, Prx6, mouse Prx1 and rat Prx1.Test Principle:The design of this assay is based on a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to human Prx1. Samples are pippetted into these wells. Nonbound Prx1 and other components of the sample should be removed by washing, then biotin-conjugated monoclonal antibody specific to Prx1 added. In order to quantitatively determine the amount of Prx1 present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) should be added to each microplate well. The final step, a TMB-substrate solution added to each well. Finally, a sulfuric acid solution is added and the resulting yellow colored product is measured at 450nm. Since the increases in absorbency is directly proportional to the amount of captured Prx1.Kit Components:Microplate: 1x96 wellsIncubation buffer: 1x30mlWashing buffer, (10x): 1x100mlStandard protein: 1 glass vial lyophilizedStandard/sample dilution buffer: 1x25mlSecond antibody: 1 glass vial lyophilizedAV-HRP, (100x): 1x150ulSecondary antibody/AV-HRP dilution buffer: 1x25mlSubstrate (TMB): 1x20mlStop solution: 1x20mlStorage and Stability:Store components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对PRDX1, Human, BioAssay ELISA Kit (Peroxiredoxin-1, Thioredoxin Peroxidase 2, Thioredoxin-dependent Peroxide Reductase 2, Proliferation-associated Gene Protein, PAG, Natural Killer Cell-enhancing Factor A, NKEF-A, TDPX2, PAGB, PAGA)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到PRDX1, Human, BioAssay ELISA Kit (Peroxiredoxin-1, Thioredoxin Peroxidase 2, Thioredoxin-dependent Peroxide Reductase 2, Proliferation-associated Gene Protein, PAG, Natural Killer Cell-enhancing Factor A, NKEF-A, TDPX2, PAGB, PAGA)产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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