- 中文名称
Penicillin, BioAssay ELISA Kit
- 英文名字
- Penicillin, BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- P3175-20
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Penicillin was accidentally detected by Alexander Fleming in 1929. The drug belongs to the mycotoxins and is generated by the mould Penicillium chrysogenum. Penicillin as an antibiotic is preferentially used in the treatment of gram-positive bacteria, both for humans and animals. Of all illegally administered drugs, antibiotics are most frequently used in productive livestock. Contaminations in food or milk are ingested by humans, and can lead to severe infections by pathogen germs which became resistant against penicillin, or to allergies. The allergic reactions appear with different severity, dependent on dose and individual disposition, and showing symptoms from urticaria to anaphylactic shock. During routine testing of milk samples for antibiotics, in more than 90% of the positive cases, betalactam preparations or penicillins are detected.The method of choice for the determination of penicillin contamination in food has always been a microbiological assay. These procedures allow however no quantitative determination and no identification of the antibiotic drug, which is achieved by a sensitive ELISA test kit or immunoaffinity columns together with HPLC.Intended Use:For the quantitative determination of penicillin in food.Sensitivity: 3ng/mlRecovery (milk): ~90%Recovery (shrimp): ~70%Incubation Time: 140 minTest Principle:The United States Biological Penicillin quantitative test is based on the principle of the enzyme linked immunosorbent assay. A penicillin conjugate is bound on the surface of a microtiter plate. Penicillin containing samples or standards and an antibody directed against penicillin are given into the wells of the microtiter plate. Immobilized and free penicillin compete for the antibody binding sites. After one hour incubation at room temperature, the wells are washed with diluted washing solution to remove unbound material. A peroxidase conjugate directed against the penicillin antibody is given into the wells and after another hour incubation, the plate is washed again. Then a substrate solution is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is inhibited by the addition of a stop solution, and the color turns yellow. The yellow color is measured photometrically at 450nm. The concentration of penicillin is indirectly proportional to the color intensity of the test sample.Kit Components:P3175-20A: Microtiter Strips, 1x96 wells each, Coated with penicillin conjugate.P3175-20B: Standard 0ng/ml ,1x1ml, ready to use.P3175-20C: Standard 4ng/ml, 1x1ml, ready to use.P3175-20D: Standard 10ng/ml, 1x1ml, ready to use.P3175-20E: Standard 40ng/ml, 1x1ml, ready to use.P3175-20F: Standard 100ng/ml, 1x1ml, ready to use.P3175-20G: Standard 400ng/ml, 1x1ml, ready to use.P3175-20H: Anti-Penicillin Antibody (mouse), 1x6ml, ready to use.P3175-20J: IgG (HRP), 1x15ml, ready to use.P3175-20K: Substrate Solution (TMB), 1x15ml, ready to use.P3175-20L: Stop Solution, 0.5M H2SO4, 1x15ml, ready to use.P3175-20M: Sample Diluent, 10X, (PBS), 2x50ml. P3175-20N: Washing Solution, 10X, (PBS + Tween-20): 1x60ml.Storage and Stability:Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Penicillin, BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Penicillin, BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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