- 中文名称
p70 S6 Kinase (Thr389, Thr421/Ser424) BioAssay Phosphorylation Detection Kit (p70 S6K) discontinued
- 英文名字
- p70 S6 Kinase (Thr389, Thr421/Ser424) BioAssay Phosphorylation Detection Kit (p70 S6K) discontinued
- 供应商
- USBiological
- 产品货号
- P1001-38
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- P70 S6 Kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (1). p70 S6 Kinase phosphorylates the 40 S subunit ribosomal protein S6 and is involved in translational control of 5´-oligopyrimidine tract mRNAs (1). A second isoform, p85 S6 Kinase, is derived from the same gene and is identical to p70 S6 Kinase except for 23 extra residues at the N-terminus that encode a nuclear localizing signal (1). Both isoforms lie on a mitogen activated signaling pathway downstream of phosphoinisitide 3-kinase (PI3K) and the target of rapamycin FRAP/mTOR; and distinct from the Ras/MAP Kinase cascade (1). The activity of p70 S6 Kinase is controlled by multiple phosphorylation events at sites located within the catalytic, linker and pseudosubstrate domains (1). Thr229 in the catalytic domain and Thr389 in the linker domain are critical for kinase function (1). However, phosphorylation at Thr389 most closely correlates p70 kinase activity in vivo (2). Prior phosphorylation of Thr389 is required for the action of 3-phosphoinositidedependent protein kinase-1 (PDK1) on Thr229 (3,4). Phosphorylation of this site is stimulated by growth factors such as insulin, EGF, FGF, serum and some Gprotein coupled receptor ligands; is completely blocked by Wortmannin, LY294002 (PI3 Kinase Inhibitors) and rapamycin (FRAP/TOR inhibitor) (1,5,6). Ser411, Thr421 and Ser424 lie within a Ser-Pro rich region located within the pseudosubstrate region (1). Phosphorylation at these sites is thought to activate Figure 1: Growth factors and cytokines activate and phosphorylate p70 S6 Kinase via Wortmannin and Rapamycin sensitive pathways that are not completely understood.Applications: Suitable for use in Western Blotting, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.Recommended Dilution:Phospho-p70 S6 Phospho-p70 S6(Thr421/Ser424) Ab (Thr389) Ab p70 S6 AbWestern blotting: 1:1000 1:1000 1:1000Immunocytochemistry (ABC): N/A N/A 1:500Immunoprecipitation: 1:250 N/A 1:250Immunohistochemistry (Paraffin): 1:50 N/A N/AOptimal dilutions to be determined by the researcher.Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- 产品特点
- 针对p70 S6 Kinase (Thr389, Thr421/Ser424) BioAssay Phosphorylation Detection Kit (p70 S6K) discontinued该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到p70 S6 Kinase (Thr389, Thr421/Ser424) BioAssay Phosphorylation Detection Kit (p70 S6K) discontinued产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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