Oxaloacetate (OAA, HOOC-CO-CH2-COOH) is a TCA cycle intermediate. It precedes citrate which is formed by the transfer of an acetyl group to OAA. OAA is formed by the deamidation of aspartate or condensation of CO2 with pyruvate or PEP. Since mammals do not possess the enzymatic machinery to form TCA cycle intermediates from acetyl CoA, OAA is one of the anaplerotic entry points via pyruvate and pyruvate carboxykinase. Oxaloacetate BioAssay Kit provides a simple, sensitive and rapid means of quantifying OAA in a variety of samples. In the assay, OAA is converted to pyruvate which is utilized to convert a nearly colorless probe to an intensely colored (lambda max = 570nm) and fluorescent (Ex/Em = 535/587nm) product. The Oxaloacetate BioAssay Kit can detect 0.1-10nmol (2-200uM) of OAA. Kit Components: 1. OAA Assay Buffer: 1x 25ml2. OAA Probe: Lyophilized3. DMSO: 1x 400ul 4. OAA Enzyme Mix: lyophilized5. Developer: lyophilized6. OAA Standard (10umol): lyophilizedStorage and Stability:Store kit at -20°C. Protect from light. Allow Assay Buffer to warm to room temperature before use. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
