- 中文名称
DAPI染色液，IHC相关试剂，用于免疫荧光实验中的细胞核复染
- 英文名字
- DAPI Stain Solution, IHC Related Reagent, For nucleus restaining in Immunofluorescence experiments
- 供应商
- Nordic BioSite
- 产品货号
- NDC-KBB-GFQWRU-50
- 产品报价
- ￥询价/50ml
- 产品说明书
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- 购买方式
- 90%产品中国有现货库存。银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- DAPI (4',6-diamidino-2-phenylindole) is a fluorescent dye which can bind DNA strands robustly, the fluorescence being detected by fluorescence microscope. DAPI can dye both live and fixed cells as it can cross intact membrane, with higher efficiency in fixed cells. The molecular formula is C16H15N5 2HCl with 350.25 g/mol molecular weight, CAS Number 28718-90-3. DAPI could pass through the cell and nucleic membranes and bind the double-strand DNA in the nucleus, producing 20 times stronger fluorescence than itself. The efficiency detected by fluorescence microscope is very high (almost 100%), having no side effects for the live cells. The sensitivity for double stranded DNA DAPI staining is many times larger comparing to ethidium bromide (EB). DAPI staining is usually used in cell death detection, as it enters more effectively and generates stronger fluorescence in dead cells. After staining with DAPI, detect with fluorescence microscope or flow cytometry. Blue fluorescent cell would be seen under the microscope after staining. The largest excitation wavelength for DAPI is 340nm (ultraviolet), and the largest emission wavelength is 488nm (blue). When DAPI binds with double-strand DNA, the largest excitation wavelength is 360nm, while the largest emission wavelength becomes 460nm. DAPI's blue emission makes it suitable for combined assays where the fluorescence ranges of DAPI and other IHC-employed fluorescent molecules like green-fluorescent fluorescein and GFP, or red-fluorescent stains like Texas Red, are completely distinctive.
- 产品描述
- DAPI（4 ′，6-二脒基-2-苯基吲哚）是一种能牢固结合DNA链的荧光染料，其荧光可通过荧光显微镜检测。DAPI可以染色活细胞和固定细胞，因为它可以穿过完整的膜，在固定细胞中具有更高的效率。分子式为C16 H15 N5 2 HCl，分子量为350.25 g/mol，CAS登记号为28718-90-3。DAPI可以穿过细胞膜和核膜，与细胞核内的双链DNA结合，产生比自身强20倍的荧光。荧光显微镜检测效率很高（几乎100%），对活细胞无副作用。与溴化乙锭（EB）相比，双链DNA DAPI染色的灵敏度大许多倍。DAPI染色通常用于细胞死亡检测，因为它更有效地进入并在死细胞中产生更强的荧光。DAPI染色后，荧光显微镜或流式细胞仪检测。染色后显微镜下可见蓝色荧光细胞。DAPI的最大激发波长为340 nm（紫外），最大发射波长为488 nm（蓝光）。当DAPI与双链DNA结合时，最大激发波长为360 nm，而最大发射波长变为460 nm。DAPI的蓝色发射使其适合于组合测定，其中DAPI和其他IHC使用的荧光分子（如绿色荧光素和GFP）或红色荧光染料（如德克萨斯红）的荧光范围完全不同。
- 产品特点
- 人CD86（T淋巴细胞活化抗原CD86）试剂盒
- 保存建议
- 该DAPI染色液，IHC相关试剂，用于免疫荧光实验中的细胞核复染产品的储存方法查看产品说明书
- 其他
- Nordic BioSite(北欧生物) 总部位于瑞典，成立于1997年，旨在分销研究和诊断领域的高质量和创新产品。 Nordic BioSite 被公认为整个北欧生物医学研究和诊断产品领域的领导者。他们拥有超过570万种的产品组合，代理了遍布欧洲和美国的顶级生物试剂制造商，是欧洲知名的一站式生物试剂代理商。除了作为分销商之外，Nordic BioSite 还拥有不断增长的自产产品线——BioSite mAb、BioSite ELISA、BioSite Flow、Optibodies和 BioSite Protein, 目前艾美捷代理的是Nordic BioSite自产产品线(共26W+产品)。Nordic BioSite的产品类型主要是抗体，蛋白和试剂盒，产品适用范围涵盖15个不同的物种(人，牛，犬，鲶鱼，鸡，海豚，马，猫，豚鼠，小鼠，绵羊，兔子，大鼠，猪和火鸡)，可为您提供一站式的生命科学研究解决方案~
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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