The Brahma protein (Brm) is an ATPase subunit of the Drosophila melanogaster Brm complex, which is highly related to the mammalian SWI/SNF chromatin-remodeling complex. Brm is a transcriptional activator of Hox genes and associates with nearly all transcriptionally active chromatin in a pattern that is non-overlapping with that of Polycomb, a repressor of Hox gene transcription. The Brm complex is an essential coactivator for the trithorax group protein Zeste, a DNA-binding activator of homeotic genes. Reduction of Brm function dramatically reduces the association of RNA polymerase II with Drosophila salivary gland chromosomes, suggesting that the chromatin remodeling activity of the Brm complex plays a general role in facilitating transcription by RNA polymerase II. Brm acts as a dominant suppressor of the rough eye phenotype that results from a hypomorphic mutation of Drosophila cyclin E by inhibiting S phase entry by acting downstream of cyclin E protein accumulation. The interaction of the Brm complex with chromatin may be modulated by BAP111, which is highly associated with the Brm complex in Drosophila embryos via an HMG domain. Brm is highly expressed in unfertilized eggs and early embryos.
应用类型
WB
免疫原
Synthetic peptide, corresponding to amino acids 1387-1432 of Human BRM.
