- 中文名称
Nitric Oxide (NO) Non-Enzymatic Assay Kit, BioAssay
- 英文名字
- Nitric Oxide (NO) Non-Enzymatic Assay Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- N2577-03
- 产品报价
- ￥1/1Kit
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The Nitric Oxide (NO) Non-Enzymatic Assay Kit allows you to measure total nitric oxide (NO) produced in in vitro experimental systems following conversion of nitrate to nitrite by metallic cadmium. Nitric oxide can be spectrophotometrically assayed by measuring the accumulation of its stable degradation products, nitrate and nitrite. The ratio of these two products in biological fluids, tissue culture media, etc. may vary substantially. Hence, for accurate assessment of the total nitric oxide generated, one must monitor both nitrate and nitrite. An excellent solution to this problem involves conversion of nitrate to nitrite catalyzed by cadmium, followed by quantitation of nitrite using Griess Reagent.This kit is suitable for the quantitative determination of total nitric oxide in samples that have high protein concentrations. The cadmium catalyst proves far more robust when in the presence of harsh deproteinating reagents than the alternative, nitrate reductase. In addition to providing all necessary components in a microtiter format, this kit employs cadmium beads, which is suitable for rapid, quantitative and inexpensive determination of the total nitric oxide. Nitrate reductase may also be used for this purpose, but cadmium is preferred for highly samples containing relatively large amounts of protein.Principle of the Assay:In aqueous solution, nitric oxide rapidly degrades to nitrate and nitrite (Equations 1 and 2). Spectrophotometric quantitation of nitrite using Griess Reagent is straightforward, but does not measure nitrate. This kit employs metallic cadmium for quantitative conversion of nitrate to nitrite (Equation 3) prior to quantitation of nitrate using Griess reagent thus providing for accurate determination of total NO production.Nitric Oxide -----------> Nitrate (Equation 1)Nitric Oxide ------------> Nitrite (Equation 2) CdNitrate -----------------> Nitrite (Equation 3)This kit can be used to accurately measure as little as 1pmol/ul (~1uM) NO produced in aqueous solutions. Very little sample is required (1 to 100ul depending on the [NO] in the sample). The completed reaction is read at 540nm.Kit Components:N2577-03A: Granulated Cadmium (Cd), 1x25g cadmium beads.N2577-03B: ZnSO4 Solution, 30% 1x2mlN2577-03C: Microcentrifuge Tubes 50x1.5ml. Use for overnight incubation of the samples.N2577-03D: Color Reagent 1, 1x7ml. Sulfanilamide (p-Aminobenzenesulfonamide) dissolved in 3N HCl.N2577-03E: Color Reagent 2, 1x7ml. N-(1-Naphthyl) ethylenediamine dihydrochloride dissolved in deionized H2O. Light Sensitive.N2577-03F: Nitrite Standard, 1x1.5ml 500pmol/ul NaNO2 (equivalent to 500uM NO).N2577-03G: Microtiter Plate, 1x96 wells. Low protein binding plate with flat-bottom wells.N2577-03H: Reagent Reservoirs 3 each. For dispensing and pipeting reagents.N2577-03J: HCl Washing Solution, 1x125ml. 0.1M HClN2577-03K: NH4OH Washing Solution, 1x125ml. 0.1M NH4OH Storage and Stability:May be stored at 4°C. Do not freeze. Stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. N2577-03E is light sensitive, keep in the dark. Cadmium should be stored in a dedicated dry environment.
- 产品特点
- 针对Nitric Oxide (NO) Non-Enzymatic Assay Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Nitric Oxide (NO) Non-Enzymatic Assay Kit, BioAssay产品后将其置于4°C Do Not Freeze保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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